Skip Navigation

Nucleic Acids Research 2004 32(20):6136-6143; doi:10.1093/nar/gkh952
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (318K) Freely available
Right arrow Supplementary Material
Right arrow Supplementary Material
Right arrow Supplementary Material
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by D'Anjou, H.
Right arrow Articles by Chartrand, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by D'Anjou, H.
Right arrow Articles by Chartrand, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 23 November 2004

Nucleic Acids Research, Vol. 32 No. 20 © Oxford University Press 2004; all rights reserved

Preferential accessibility to specific genomic loci for the repair of double-strand breaks in human cells

Hélène D'Anjou1, Catherine Chabot1,3 and Pierre Chartrand1,2,3,4,*

1 Molecular Biology Program and 2 Department of Pathology and Cellular Biology, 3 Montreal Cancer Institute, CHUM and 4 IRIC, Université de Montréal, Montréal, Québec, Canada

* To whom correspondence should be addressed. Tel: +1 514 343 6111, Ext. 3573; Fax: +1 514 343 7780; Email: pierre.chartrand{at}umontreal.ca

Received July 16, 2004; Revised October 8, 2004; Accepted November 1, 2004

The dynamic organization of the human genome in the nucleus is gaining recognition as a determining factor in its functional regulation. In order to be expressed, replicated or repaired, a genomic locus has to be present at the right place at the right time. In the present study, we have investigated the choice of a double-strand break (DSB) repair partner for a given genomic loci in an ATM-deficient human fibroblast cell line. We found that partner choice is restricted such that a given genomic locus preferentially uses certain sites in the genome to repair itself. These preferential sites can be in the vicinity of the damage site or megabases away or on other chromosomes entirely, while potential sites closer to the break along the length of the chromosome can be ignored. Moreover, there can be more than a 10-fold difference in usage between repair sites located only 10 kb apart. Interestingly, arms of a given chromosome are less accessible to one another than to other chromosomes. Altogether, these results indicate that the accessibility between genomic sites in the human genome during DSB repair is specific and conserved in a cell population.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Natl Cancer Inst MonogrHome page
E. Soutoglou and T. Misteli
On the Contribution of Spatial Genome Organization to Cancerous Chromosome Translocations
J Natl Cancer Inst Monographs, July 1, 2008; 2008(39): 16 - 19.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Schildkraut, C. A. Miller, and J. A. Nickoloff
Transcription of a Donor Enhances Its Use during Double-Strand Break-Induced Gene Conversion in Human Cells
Mol. Cell. Biol., April 15, 2006; 26(8): 3098 - 3105.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.