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Nucleic Acids Research 2004 32(20):e161; doi:10.1093/nar/gnh158
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Published online 23 November 2004

Nucleic Acids Research, Vol. 32 No. 20 © Oxford University Press 2004; all rights reserved

Bigenic Cre/loxP, pu{Delta}tk conditional genetic ablation

You-Tzung Chen1, Regis Levasseur1, Sukeshi Vaishnav1, Gerard Karsenty1 and Allan Bradley1,2,*

1 Program in Developmental Biology, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA and 2 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

* To whom correspondence should be addressed. Tel: +44 1223 494881; Fax: +44 1223 494714; Email: abradley{at}sanger.ac.uk

Received October 13, 2004; Revised and Accepted October 29, 2004

Genetic ablation experiments are used to resolve problems regarding cell lineages and the in vivo function of certain groups of cells. We describe a two-component conditional ablation technology using a mouse carrying an X-linked pu{Delta}tk transgene, which is only activated in cells expressing Cre. Ablation of the Cre-expressing cells can be temporally regulated by the time of ganciclovir (GCV) administration. This strategy was demonstrated using a Col2Cre transgenic line. Differentiating chondrocytes in bigenic animals could be ablated at different developmental stages resulting in disorganized growth plates and dwarfism. Macrocephaly, macroglossia and umbilical hernia were also observed in ablated 18.5 dpc embryos. Crosses between the pu{Delta}tk selector transgenic line and existing cre lines will facilitate numerous temporally regulated tissue-specific ablation experiments.


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