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Published online 11 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 3 e28
© 2004 Oxford University Press

Semiconductor nanocrystal probes for human metaphase chromosomes

Yan Xiao and Peter E. Barker*,1

GeoCenters, Inc. and DNA Technologies Group, and 1 NIST-NCI Biomarkers Validation Laboratory, DNA Technologies Group, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 831, Gaithersburg, MD 20899-8311, USA

*To whom correspondence should be addressed. Tel: +1 301 975 5402; Fax: +1 301 975 8967; Email: peter.barker{at}nist.gov

To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photostable than organic fluorophores Texas Red and fluorescein. Thus, semiconductor nanocrystal fluorophores offer a more stable and quantitative mode of fluorescence in situ hybridization (FISH) for research and clinical applications.


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