Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (490K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ferguson, A.
Right arrow Articles by Cload, S. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ferguson, A.
Right arrow Articles by Cload, S. T.
Related Collections
Right arrow RNA characterisation and manipulation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 16 March 2004

Nucleic Acids Research, 2004, Vol. 32, No. 5 1756-1766
© 2004 Oxford University Press

A novel strategy for selection of allosteric ribozymes yields RiboReporterTM sensors for caffeine and aspartame

Alicia Ferguson, Ryan M. Boomer, Markus Kurz, Sara C. Keene, John L. Diener, Anthony D. Keefe, Charles Wilson and Sharon T. Cload*

Archemix Corporation, 1 Hampshire Street, Cambridge, MA 02139, USA

*To whom correspondence should be addressed. Tel: +1 617 475 2333; Fax: +1 617 225 2855; Email: cload{at}archemix.com

We have utilized in vitro selection technology to develop allosteric ribozyme sensors that are specific for the small molecule analytes caffeine or aspartame. Caffeine- or aspartame-responsive ribozymes were converted into fluorescence-based RiboReporterTM sensor systems that were able to detect caffeine or aspartame in solution over a concentration range from 0.5 to 5 mM. With read-times as short as 5 min, these caffeine- or aspartame-dependent ribozymes function as highly specific and facile molecular sensors. Interestingly, successful isolation of allosteric ribozymes for the analytes described here was enabled by a novel selection strategy that incorporated elements of both modular design and activity-based selection methods typically used for generation of catalytic nucleic acids.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Alper, C. Fischer, E. Nevoigt, and G. Stephanopoulos
Tuning genetic control through promoter engineering
PNAS, September 6, 2005; 102(36): 12678 - 12683.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.