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Published online 23 April 2004

Nucleic Acids Research, 2004, Vol. 32, No. 7 e65
© 2004 Oxford University Press

Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates

Deirdre Murphy, Ramon Eritja1 and Gareth Redmond*

Nanotechnology Group, NMRC, Lee Maltings, Cork, Ireland and 1 Department of Structural Biology, Instituto de Biología Molecular de Barcelona, CSIC, Jordi Girona 18-26, E-08034 Barcelona, Spain

*To whom correspondence should be addressed. Tel: +353 21 4904077; Fax: +353 21 4270271; Email: gareth.redmond{at}nmrc.ie

Received February 6, 2004; Revised March 10, 2004; Accepted March 29, 2004

Gold nanoparticle labels, combined with UV-visible optical absorption spectroscopic methods, are employed to probe the temperature-dependent solution properties of DNA triple helices. By using oligonucleotide–nanoparticle conjugates to characterize triplex denaturation, for the first time triplex to duplex melting transitions may be sensitively monitored, with minimal signal interference from duplex to single strand melting, for both parallel and antiparallel triple helices. Further, the comparative sequence-dependent stability of DNA triple helices may also be examined using this approach. Specifically, triplex to duplex melting transitions for triplexes formed using oligonucleotides that incorporate 8-aminoguanine derivatives were successfully monitored and stabilization of both parallel and antiparallel triplexes following 8-aminoguanine substitutions is demonstrated.


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