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Published online 25 May 2004

Nucleic Acids Research, 2004, Vol. 32, No. 9 2947-2956
© 2004 Oxford University Press

Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors

Ian M. Fingerman, Kristina Sutphen, Sherwin P. Montano1, Millie M. Georgiadis1 and Andrew K. Vershon*

Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA and 1 Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA

*To whom correspondence should be addressed at Waksman Institute, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA. Tel: +1 732 445 2905; Fax: +1 732 445 5735; Email: vershon{at}waksman.rutgers.edu

Received December 5, 2003; Revised March 30, 2004;; Accepted May 6, 2004

The Ndt80 protein of the yeast Saccharomyces cerevisiae is the founding member of a new sub-family of proteins in the Ig-fold superfamily of transcription factors. The crystal structure of Ndt80 bound to DNA shows that it makes contacts through several loops on one side of the protein that connect ß-strands which form the ß-sandwich fold common to proteins in this superfamily. However, the DNA-binding domain of Ndt80 is considerably larger than many other members of the Ig-fold superfamily and it appears to make a larger number of contacts with the DNA than these proteins. To determine the contribution of each of these contacts and to examine if the mechanism of Ndt80 DNA binding was similar to other members of the Ig-fold superfamily, amino acid substitutions were introduced at each residue that contacts the DNA and assayed for their effect on Ndt80 activity. Many of the mutations caused significant decreases in DNA-binding affinity and transcriptional activation. Several of these are in residues that are not found in other sub-families of Ig-fold proteins. These additional contacts are likely responsible for Ndt80’s ability to bind DNA as a monomer while most other members require additional domains or cofactors to recognize their sites.


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