Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (49K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by To, C.
Right arrow Articles by Stanford, W. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by To, C.
Right arrow Articles by Stanford, W. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2004, Vol. 32, Database issue D557-D559
© 2004 Oxford University Press

The Centre for Modeling Human Disease Gene Trap resource

Christine To1, Trevor Epp1,2, Tammy Reid1,2, Qing Lan1,2, Mei Yu1,2, Carol Y. J. Li1,2, Minako Ohishi1,2, Paula Hant1, Nora Tsao1, Guillermo Casallo1,3, Janet Rossant1,4, Lucy R. Osborne1,3,4 and William L. Stanford*,1,2,5

1 Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada, 2 Institute of Biomaterials and Biomedical Engineering, 3 Department of Medicine, 4 Department of Molecular and Medical Genetics and 5 Institute of Medical Science, University of Toronto, Canada

*To whom correspondence should be addressed at Institute of Biomaterials and Biomedical Engineering, University of Toronto, 4 Taddle Creek Road, Room 407, Rosebrugh Building, Toronto, Ontario M5S 3G9, Canada. Tel: +1 416 946 8379; Fax: +1 416 978 4317; Email: william.stanford{at}utoronto.ca
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

Gene trap mutagenesis of mouse embryonic stem cells generates random loss-of-function mutations, which can be identified by a sequence tag and can often report the endogenous expression of the mutated gene. The Centre for Modeling Human Disease is performing expression- and sequence-based screens of gene trap insertions to generate new mouse mutations as a resource for the scientific community. The gene trap insertions are screened using multiplexed in vitro differentiation and induction assays, and sequence tags are generated to complement expression profiles. Researchers may search for insertions in genes expressed in target cell lineages, under specific in vitro conditions, or based upon sequence identity via an online searchable database (http://www.cmhd.ca/sub/genetrap.asp). The clones are available as a resource to researchers worldwide to help to functionally annotate the mammalian genome and will serve as a source to test candidate loci identified by phenotype-driven mutagenesis screens.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DMMHome page
D. Nguyen and T. Xu
The expanding role of mouse genetics for understanding human biology and disease
Dis. Model. Mech., July 1, 2008; 1(1): 56 - 66.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Roma, M. Sardiello, G. Cobellis, P. Cruz, G. Lago, R. Sanges, and E. Stupka
The UniTrap resource: tools for the biologist enabling optimized use of gene trap clones
Nucleic Acids Res., January 11, 2008; 36(suppl_1): D741 - D746.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. S. Nord, P. J. Chang, B. R. Conklin, A. V. Cox, C. A. Harper, G. G. Hicks, C. C. Huang, S. J. Johns, M. Kawamoto, S. Liu, et al.
The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse
Nucleic Acids Res., January 1, 2006; 34(suppl_1): D642 - D648.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Shigeoka, M. Kawaichi, and Y. Ishida
Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells
Nucleic Acids Res., February 1, 2005; 33(2): e20 - e20.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Hirashima, A. Bernstein, W. L. Stanford, and J. Rossant
Gene-trap expression screening to identify endothelial-specific genes
Blood, August 1, 2004; 104(3): 711 - 718.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.