ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry
W.M. Keck Center for Comparative and Functional Genomics and 1 Department of Chemistry, University of Illinois, Urbana, IL, 61801, USA and 2 EMBL Outstation–EBI, Hinxton, Cambridge CB10 1SD, UK
* To whom correspondence should be addressed. Tel: +1 217 244 3927; Fax: +1 217 244 8068; Email: kelleher{at}scs.uiuc.edu
Received February 16, 2004; Revised and Accepted April 20, 2004
ProSight PTM (https://prosightptm.scs.uiuc.edu/) is a web application for identification and characterization of proteins using mass spectra data from ‘top-down’ fragmentation of intact protein ions (i.e. without any tryptic digestion). ProSight PTM has many tools and graphical features to facilitate analysis of single proteins, proteins in mixtures and proteins fragmented in parallel. Sequence databases from across the phylogenetic tree are supported, with a new database strategy of ‘shotgun annotation’ used to assist characterization of wild-type proteins. During a database search, data from divergent sources regarding potential mass differences such as polymorphisms, alternate splicing and post-translational modifications are utilized. The user can optionally control how much of this biological variability should be searched.
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