Skip Navigation

Nucleic Acids Research 2005 33(1):347-355; doi:10.1093/nar/gki183
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1686K) Freely available
Right arrow Screen PDF (398K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tops, B. B. J.
Right arrow Articles by Ketting, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tops, B. B. J.
Right arrow Articles by Ketting, R. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 13 January 2005

© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions{at}oupjournals.org.

Article

RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans

Bastiaan B. J. Tops, Hiroaki Tabara1, Titia Sijen, Femke Simmer, Craig C. Mello1, Ronald H. A. Plasterk* and René F. Ketting

Hubrecht Laboratory, Centre for Biomedical Genetics Uppsalalaan 8, 3584 CT Utrecht, The Netherlands 1 Department of Cell Biology, Program in Molecular Medicine, University of Massachusetts Cancer Centre Worcester, MA 01605, USA

*To whom correspondence should be addressed. Tel: +31 30 212 1963; Fax: +31 30 251 6554; Email: plasterk{at}niob.knaw.nl

Received December 7, 2004. Revised December 21, 2004. Accepted December 21, 2004.

In Caenorhabditis elegans, the activity of transposable elements is repressed in the germline. One of the mechanisms involved in this repression is RNA interference (RNAi), a process in which dsRNA targets cleavage of mRNAs in a sequence-specific manner. The first gene found to be involved in RNAi and transposon silencing in C.elegans is mut-7, a gene encoding a putative exoribonuclease. Here, we show that the MUT-7 protein resides in complexes of ~250 kDa in the nucleus and in the cytosol. In addition, we find that upon triggering of RNAi the cytosolic MUT-7 complex increases in size. This increase is independent of the presence of target RNA, but does depend on the presence of RDE-1 and RDE-4, two proteins involved in small interfering RNA (siRNA) production. Finally, using a yeast two-hybrid screen, we identified RDE-2/MUT-8 as one of the other components of this complex. This protein is encoded by the rde-2/mut-8 locus, previously implicated in RNAi and transposon silencing. Using genetic complementation analysis, we show that the interaction between these two proteins is required for efficient RNAi in vivo. Together these data support a role for the MUT-7/RDE-2 complex downstream of siRNA formation, but upstream of siRNA mediated target RNA recognition, possibly indicating a role in the siRNA amplification step.

Correspondence may also be addressed to René F. Ketting. Tel: +31 30 212 1964; Fax: +31 30 251 6554; Email: ketting{at}niob.knaw.nl

Present address: Hiroaki Tabara, Kyoto University, Graduate School of Medicine, HMRO, Yoshida-Konoe, Sakyo, Kyoto 606-8501, Japan


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
M. C. K. Leung, P. L. Williams, A. Benedetto, C. Au, K. J. Helmcke, M. Aschner, and J. N. Meyer
Caenorhabditis elegans: An Emerging Model in Biomedical and Environmental Toxicology
Toxicol. Sci., November 1, 2008; 106(1): 5 - 28.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
W. Han, P. Sundaram, H. Kenjale, J. Grantham, and L. Timmons
The Caenorhabditis elegans rsd-2 and rsd-6 Genes Are Required for Chromosome Functions During Exposure to Unfavorable Environments
Genetics, April 1, 2008; 178(4): 1875 - 1893.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. Sundaram, W. Han, N. Cohen, B. Echalier, J. Albin, and L. Timmons
Caenorhabditis elegans ABCRNAi Transporters Interact Genetically With rde-2 and mut-7
Genetics, February 1, 2008; 178(2): 801 - 814.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A.-J. Rodrigues, G. Coppola, C. Santos, M. d. C. Costa, M. Ailion, J. Sequeiros, D. H. Geschwind, and P. Maciel
Functional genomics and biochemical characterization of the C. elegans orthologue of the Machado-Joseph disease protein ataxin-3
FASEB J, April 1, 2007; 21(4): 1126 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Sundaram, B. Echalier, W. Han, D. Hull, and L. Timmons
ATP-binding Cassette Transporters Are Required for Efficient RNA Interference in Caenorhabditis elegans
Mol. Biol. Cell, August 1, 2006; 17(8): 3678 - 3688.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
R. C. LEE, C. M. HAMMELL, and V. AMBROS
Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans
RNA, April 1, 2006; 12(4): 589 - 597.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
B.B.J. TOPS, R.H.A. PLASTERK, and R.F. KETTING
The Caenorhabditis elegans Argonautes ALG-1 and ALG-2: Almost Identical yet Different
Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 189 - 194.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
V. J.P. Robert, T. Sijen, J. van Wolfswinkel, and R. H.A. Plasterk
Chromatin and RNAi factors protect the C. elegans germline against repetitive sequences
Genes & Dev., April 1, 2005; 19(7): 782 - 787.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.