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Nucleic Acids Research 2005 33(1):e7; doi:10.1093/nar/gni006
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Published online 13 January 2005

© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions{at}oupjournals.org.

Methods Online

Conditional UAS-targeted repression in Drosophila

Alberto Pascual, Kai-Lian Huang and Thomas Preat*

Développement, Evolution, Plascticité du Système Nerveux, C.N.R.S. 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France

*To whom correspondence should be addressed. Tel: +33 1 69 82 36 38; Email: preat{at}iaf.cnrs-gif.fr

Received September 23, 2004. Revised December 13, 2004. Accepted December 13, 2004.

The Gal4–UAS enhancer trap system is useful for driving gene expression in various tissues. A new tool that extends Gal4 technology is described here. A fusion protein containing the Gal4 binding domain and the repression domain of the isolator suppressor of hairy wing was placed under the control of a heat shock-inducible promoter. The construct mediates the conditional repression of genes located downstream of a UAS sequence. The repressive effects of the chimeric protein on fasII gene expression were tested by western-blot analysis and in brain sections of adult Drosophila. Owing to the increasing number of Gal4 and UAS transgenic lines, this versatile system will facilitate the study of gene function.

Present address: Alberto Pascual, Laboratorio de Investigaciones Biomédicas, Hospital Universitario Virgen del Rocívio, 41013 Sevilla, Spain


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