Skip Navigation

Nucleic Acids Research 2005 33(10):3185-3192; doi:10.1093/nar/gki632
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (873K) Freely available
Right arrow Screen PDF (223K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (34)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Qi, J.
Right arrow Articles by Shafer, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qi, J.
Right arrow Articles by Shafer, R. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 2 June 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Covalent ligation studies on the human telomere quadruplex

Jianying Qi and Richard H. Shafer*

Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco, CA 94143-0446, USA

*To whom correspondence should be addressed. Tel: +1 415 476 2761; Fax: +1 415 476 0688; Email: shafer{at}cgl.ucsf.edu

Received April 4, 2005. Revised May 15, 2005. Accepted May 15, 2005.

Recent X-ray crystallographic studies on the human telomere sequence d[AGGG(TTAGGG)3] revealed a unimolecular, parallel quadruplex structure in the presence of potassium ions, while earlier NMR results in the presence of sodium ions indicated a unimolecular, antiparallel quadruplex. In an effort to identify and isolate the parallel form in solution, we have successfully ligated into circular products the single-stranded human telomere and several modified human telomere sequences in potassium-containing solutions. Using these sequences with one or two terminal phosphates, we have made chemically ligated products via creation of an additional loop. Circular products have been identified by polyacrylamide gel electrophoresis, enzymatic digestion with exonuclease VII and electrospray mass spectrometry in negative ion mode. Optimum pH for the ligation reaction of the human telomere sequence ranges from 4.5 to 6.0. Several buffers were also examined, with MES yielding the greatest ligation efficiency. Human telomere sequences with two phosphate groups, one each at the 3' and 5' ends, were more efficient at ligation, via pyrophosphate bond formation, than the corresponding sequences with only one phosphate group, at the 5' end. Circular dichroism spectra showed that the ligation product was derived from an antiparallel, single-stranded guanine quadruplex rather than a parallel single-stranded guanine quadruplex structure.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
K. W. Lim, P. Alberti, A. Guedin, L. Lacroix, J.-F. Riou, N. J. Royle, J.-L. Mergny, and A. T. Phan
Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G{middle dot}C{middle dot}G{middle dot}C tetrad
Nucleic Acids Res., October 1, 2009; 37(18): 6239 - 6248.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Renciuk, I. Kejnovska, P. Skolakova, K. Bednarova, J. Motlova, and M. Vorlickova
Arrangements of human telomere DNA quadruplex in physiologically relevant K+ solutions
Nucleic Acids Res., October 1, 2009; 37(19): 6625 - 6634.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Bogani, C. N. Chua, and P. E. Boehmer
Reconstitution of Uracil DNA Glycosylase-initiated Base Excision Repair in Herpes Simplex Virus-1
J. Biol. Chem., June 19, 2009; 284(25): 16784 - 16790.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Y. Lee and D. S. Kim
Dramatic effect of single-base mutation on the conformational dynamics of human telomeric G-quadruplex
Nucleic Acids Res., June 1, 2009; 37(11): 3625 - 3634.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. I. Gaynutdinov, R. D. Neumann, and I. G. Panyutin
Structural polymorphism of intramolecular quadruplex of human telomeric DNA: effect of cations, quadruplex-binding drugs and flanking sequences
Nucleic Acids Res., July 1, 2008; 36(12): 4079 - 4087.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. T. Phan, V. Kuryavyi, K. N. Luu, and D. J. Patel
Structure of two intramolecular G-quadruplexes formed by natural human telomere sequences in K+ solution
Nucleic Acids Res., October 8, 2007; 35(19): 6517 - 6525.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Dai, M. Carver, C. Punchihewa, R. A. Jones, and D. Yang
Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence
Nucleic Acids Res., August 1, 2007; 35(15): 4927 - 4940.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C.-C. Chang, C.-W. Chien, Y.-H. Lin, C.-C. Kang, and T.-C. Chang
Investigation of spectral conversion of d(TTAGGG)4 and d(TTAGGG)13 upon potassium titration by a G-quadruplex recognizer BMVC molecule
Nucleic Acids Res., May 14, 2007; 35(9): 2846 - 2860.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Dai, C. Punchihewa, A. Ambrus, D. Chen, R. A. Jones, and D. Yang
Structure of the intramolecular human telomeric G-quadruplex in potassium solution: a novel adenine triple formation
Nucleic Acids Res., April 19, 2007; (2007) gkm009v2.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. T. Phan, K. N. Luu, and D. J. Patel
Different loop arrangements of intramolecular human telomeric (3+1) G-quadruplexes in K+ solution
Nucleic Acids Res., November 14, 2006; 34(19): 5715 - 5719.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Ambrus, D. Chen, J. Dai, T. Bialis, R. A. Jones, and D. Yang
Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution.
Nucleic Acids Res., January 1, 2006; 34(9): 2723 - 2735.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Y. Lee, B. Okumus, D. S. Kim, and T. Ha
Extreme conformational diversity in human telomeric DNA
PNAS, December 27, 2005; 102(52): 18938 - 18943.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Li, J. J. Correia, L. Wang, J. O. Trent, and J. B. Chaires
Not so crystal clear: the structure of the human telomere G-quadruplex in solution differs from that present in a crystal
Nucleic Acids Res., August 16, 2005; 33(14): 4649 - 4659.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.