Skip Navigation

Nucleic Acids Research 2005 33(12):3828-3836; doi:10.1093/nar/gki695
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1030K) Freely available
Right arrow Screen PDF (304K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kempeneers, V.
Right arrow Articles by Herdewijn, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kempeneers, V.
Right arrow Articles by Herdewijn, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 12 July 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization

Veerle Kempeneers, Marleen Renders, Matheus Froeyen and Piet Herdewijn*

Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium

*To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +32 16 337340; Email: Piet.Herdewijn{at}rega.kuleuven.ac.be

Received April 11, 2005. Revised May 27, 2005. Accepted June 17, 2005.

DNA polymerases from different evolutionary families [Vent (exo–) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo–) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Kuwahara, S. Obika, J.-i. Nagashima, Y. Ohta, Y. Suto, H. Ozaki, H. Sawai, and T. Imanishi
Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides
Nucleic Acids Res., August 1, 2008; 36(13): 4257 - 4265.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Robeyns, P. Herdewijn, and L. Van Meervelt
Influence of the incorporation of a cyclohexenyl nucleic acid (CeNA) residue onto the sequence d(CGCGAATTCGCG)
Nucleic Acids Res., March 1, 2008; 36(5): 1407 - 1414.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.