Skip Navigation

Nucleic Acids Research 2005 33(12):e106; doi:10.1093/nar/gni109
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1293K) Freely available
Right arrow Screen PDF (556K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hong, B. J.
Right arrow Articles by Park, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hong, B. J.
Right arrow Articles by Park, J. W.
Related Collections
Right arrow Microarray
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 7 July 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Methods Online

DNA microarrays on nanoscale-controlled surface

Bong Jin Hong, Vijaya Sunkara and Joon Won Park*

Department of Chemistry, Center for Integrated Molecular Systems, Division of Molecular and Life Sciences, Pohang University of Science and Technology Pohang 790-784, Korea

*To whom correspondence should be addressed. Tel: +82 54 279 2119; Fax: +82 54 279 0653; Email: jwpark{at}postech.ac.kr

Received April 24, 2005. Revised June 21, 2005. Accepted June 21, 2005.

We have developed new surface to ensure a proper spacing between immobilized biomolecules. While DNA microarray on this surface provided each probe DNA with ample space for hybridization with incoming target DNAs, the microarray showed enhanced discrimination efficiency for various types of single nucleotide polymorphism. The high discrimination efficiency holds for all tested cases (100:<1 for internal mismatched cases; 100:<28 for terminal mismatched ones). In addition, by investigating influence of hybridization temperature and washing condition on the fluorescence intensity and the discrimination efficiency with and without controlled mesospacing, it was observed that the nanoscale-controlled surface showed good discrimination efficiency in a wide range of temperature (37–50°C), and hybridization behavior on the surface was in agreement with the solution one. Intriguingly, it was found that washing process after the hybridization was critical for the high discrimination efficiency. For the particular case, washing process was so efficient that only 30 s washing was sufficient to reach the optimal discrimination ratio.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
L. Poulsen, M. J. Soe, D. Snakenborg, L. B. Moller, and M. Dufva
Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
Nucleic Acids Res., November 1, 2008; 36(20): e132 - e132.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Pasternak, E. Kierzek, K. Pasternak, D. H. Turner, and R. Kierzek
A chemical synthesis of LNA-2,6-diaminopurine riboside, and the influence of 2'-O-methyl-2,6-diaminopurine and LNA-2,6-diaminopurine ribosides on the thermodynamic properties of 2'-O-methyl RNA/RNA heteroduplexes
Nucleic Acids Res., June 12, 2007; (2007) gkm421v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. E. Pozhitkov, R. D. Stedtfeld, S. A. Hashsham, and P. A. Noble
Revision of the nonequilibrium thermal dissociation and stringent washing approaches for identification of mixed nucleic acid targets by microarrays
Nucleic Acids Res., May 14, 2007; 35(9): e70 - e70.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Cattaruzza, A. Cricenti, A. Flamini, M. Girasole, G. Longo, T. Prosperi, G. Andreano, L. Cellai, and E. Chirivino
Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination of the surface coverage and of the hybridization efficiency; parallel imaging of the process by Atomic Force Microscopy
Nucleic Acids Res., February 28, 2006; 34(4): e32 - e32.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.