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Nucleic Acids Research 2005 33(13):e114; doi:10.1093/nar/gni114
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Published online 21 July 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

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Four new type I restriction enzymes identified in Escherichia coli clinical isolates

Julie K. A. Kasarjian, Yoshiaki Kodama, Masatake Iida, Katsura Matsuda1 and Junichi Ryu*

Division of Microbiology and Molecular Genetics, Department of Biochemistry and Microbiology, Loma Linda University Loma Linda, CA 92350, USA 1Department of Earth and Biological Sciences, Loma Linda University Loma Linda, CA 92350, USA

*To whom correspondence should be addressed. Tel: +1 909 558 1000, Ext. 42773; Fax: +1 909 558 4035; Email: jryu{at}llu.edu

Received May 26, 2005. Revised June 30, 2005. Accepted June 30, 2005.

Using a plasmid transformation method and the RM search computer program, four type I restriction enzymes with new recognition sites and two isoschizomers (EcoBI and Eco377I) were identified in a collection of clinical Escherichia coli isolates. These new enzymes were designated Eco394I, Eco826I, Eco851I and Eco912I. Their recognition sequences were determined to be GAC(5N)RTAAY, GCA(6N)CTGA, GTCA(6N)TGAY and CAC(5N)TGGC, respectively. A methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the adenines that prevent cleavage when methylated (underlined). These results suggest that type I enzymes are abundant in E.coli and many other bacteria, as has been inferred from bacterial genome sequencing projects.

Present addresses: Yoshiaki Kodama, Department of Orthopedic Surgery, Tokyo Metropolitan Fuchu Hospital, 2-9-2 Musashidai, Fuchu-shi, Tokyo, Japan

Masatake Iida, Department of Gastroenterological Surgery, School of Medicine, Akita University, Akita, Japan


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J. Ryu and E. Rowsell
Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids
Nucleic Acids Res., August 1, 2008; 36(13): e81 - e81.
[Abstract] [Full Text] [PDF]


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