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Nucleic Acids Research 2005 33(14):4519-4526; doi:10.1093/nar/gki764
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Published online 9 August 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Evidence for rolling circle replication of tandem genes in Drosophila

Sarit Cohen*, Neta Agmon, Keren Yacobi, Margarita Mislovati and Daniel Segal

Department of Molecular Microbiology and Biotechnology, Tel-Aviv University Tel Aviv 69978, Israel

*To whom correspondence should be addressed. Tel: +972 3 6407530; Fax: +972 3 6409407; Email: scohen{at}post.tau.ac.il

Received June 9, 2005. Revised July 26, 2005. Accepted July 26, 2005.

Extrachromosomal circular DNA (eccDNA) is one characteristic of the plasticity of the eukaryotic genome. It is found in various organisms and contains sequences derived primarily from repetitive chromosomal DNA. Using 2D gel electrophoresis, we have previously detected eccDNA composed of chromosomal tandem repeats throughout the life cycle of Drosophila. Here, we report for the first time evidence suggesting the occurrence of rolling circle replication of eccDNA in Drosophila. We show, on 2D gels, specific structures that can be enriched by benzoylated naphthoylated DEAE-cellulose chromatography and were identified in other systems as rolling circle intermediates (RCIs). These RCIs are homologous to histone genes, Stellate and Suppressor of Stellate, which are all organized in the chromosomes as tandem repeats. RCIs are detected throughout the life cycle of Drosophila and in cultured fly cells. These structures are found regardless of the expression of the replicated gene or of its chromosomal copy number.

Present address: Keren Yacobi, Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel


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