Published online 26 August 2005
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Interaction of Staufen1 with the 5' end of mRNA facilitates translation of these RNAs
1Département de Biochimie, Université de Montréal Montréal, QC, Canada 2Centre de Recherche en Sciences Neurologiques, Université de Montréal Montréal, QC, Canada 3Lady Davis Institute for Medical Research, McGill University Montréal, Canada H3C 3J7
*To whom correspondence should be addressed at Department of Biochemistry, University of Montreal, PO Box 6128, Station Centre Ville, Montreal, QC, Canada H3C 3J7. Tel: +1 514 343 5802; Fax: +1 514 343 2210; Email: luc.desgroseillers{at}umontreal.ca
Received June 22, 2005. Revised August 9, 2005. Accepted August 9, 2005.
Staufen1 is a component of transported ribonucleoprotein complexes. Genetic work in Drosophila has suggested that Staufen plays a role in the de-repression of translation of oskar mRNA following localization. To determine whether Staufen1 can play a similar role in mammals, we studied translation of transcripts in the presence or in the absence of Staufen1. Translationally repressed mRNAs were generated by fusing the structured human immunodeficiency virus type 1 trans-activating response (TAR) element to the 5' end of a reporter transcript. In rabbit reticulocyte lysates and in mammalian cultured cells, the addition of Staufen1 resulted in the up-regulation of reporter activity when translation was driven by the TAR-bearing RNA. In contrast, Staufen1 had no effect on translation of efficiently translated mRNAs lacking an apparent structured 5' end, suggesting that Staufen1-binding to the 5' end is required for enhanced translation. Consistently, Staufen1 RNA-binding activity is necessary for this translational effect. In addition, similar up-regulation of translation was observed when Staufen1 was tethered to the 5' end of mRNAs via other structured RNAs, the highest level of translational increase being obtained with the bona fide Staufen1-binding site of the Arf1 transcript. The expression of Staufen1 promoted polysomal loading of TAR-luciferase transcripts resulting in enhanced translation. Our results support a model in which the expression of Staufen1 and its interaction with the 5' end of RNA and ribosomes facilitate translation initiation.
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
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