Skip Navigation

Nucleic Acids Research 2005 33(16):5139-5144; doi:10.1093/nar/gki802
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (4794K) Freely available
Right arrow Screen PDF (488K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lux, C.
Right arrow Articles by Eick, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lux, C.
Right arrow Articles by Eick, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 12 September 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Molecular Biology

Transition from initiation to promoter proximal pausing requires the CTD of RNA polymerase II

C. Lux, H. Albiez1, R. D. Chapman, M. Heidinger, M. Meininghaus, R. Brack-Werner2, A. Lang, M. Ziegler2, T. Cremer1 and D. Eick*

Institute of Clinical Molecular Biology and Tumour Genetics, GSF-National Research Center for Environment and Health Marchioninistrasse 25, D-81377 Munich, Germany 1Department of Biology II, Ludwig-Maximilians University Munich D-82152 Planegg-Martinsried, Germany 2Institute of Molecular Virology, GSF-National Research Center for Environment and Health Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany

*To whom correspondence should be addressed. Tel: +49 89 7099512; Fax: +49 89 7099500; Email: eick{at}gsf.de

Received July 28, 2005. Revised August 15, 2005. Accepted August 15, 2005.

The C-terminal domain (CTD) of mammalian RNA polymerase II consists of 52 repeats of the consensus hepta-peptide YSPTSPS, and links transcription to the processing of pre-mRNA. Although Pol II with a CTD shortened to five repeats (Pol II {Delta}5) is transcriptionally inactive on chromatin templates, it is not clear whether CTD is required for promoter recognition in vivo. Here, we demonstrate that in the context of chromatin, Pol II {Delta}5 can bind to the c-myc promoter with the same efficiency as wild type Pol II. However, Pol II {Delta}5 does not form a stable initiation complex, and does not transcribe promoter proximal sequences. Fluorescence recovery after photobleaching (FRAP) experiments with cells expressing enhanced green fluorescent protein (EGFP)-tagged {Delta}5 or wildtype Pol II revealed a single, highly mobile Pol II {Delta}5 fraction whereas wildtype Pol II yielded less mobile fractions. These data suggest that CTD is not required for promoter recognition, but rather for subsequent formation of a stable initiation complex and isomerization to an elongation competent complex.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.