Published online 12 September 2005
Molecular Biology |
Methylation patterns of histone H3 Lys 4, Lys 9 and Lys 27 in transcriptionally active and inactive Arabidopsis genes and in atx1 mutants
School of Biological Sciences, UNL Lincoln, NE 68588-011, USA
*To whom correspondence should be addressed. Tel: +1 402 4723993; Fax: +1 402 4722083; Email: zavramova2{at}unl.edu
Received June 23, 2005. Revised August 1, 2005. Accepted August 25, 2005.
Covalent modifications of histone-tail amino acid residues communicate information via a specific ‘histone code’. Here, we report histone H3-tail lysine methylation profiles of several Arabidopsis genes in correlation with their transcriptional activity and the input of the epigenetic factor ARABIDOPSIS HOMOLOG OF TRITHORAX (ATX1) at ATX1-regulated loci. By chromatin immunoprecipitation (ChIP) assays, we compared modification patterns of a constitutively expressed housekeeping gene, of a tissue-specific gene, and among genes that differed in degrees of transcriptional activity. Our results suggest that the di-methylated isoform of histone H3-lysine4 (m2K4/H3) provide a general mark for gene-related sequences distinguishing them from non-transcribed regions. Lys-4 (K4/H3), lys-9 (K9/H3) and lys-27 (K27/H3) nucleosome methylation patterns of plant genes may be gene-, tissue- or development-regulated. Absence of nucleosomes from the LTP-promotor was not sufficient to provoke robust transcription in mutant atx1-leaf chromatin, suggesting that the mechanism repositioning nucleosomes at transition to flowering functioned independently of ATX1.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Li, K. Wu, G. Fu, Y. Li, Y. Zhong, X. Lin, Y. Zhou, L. Tian, and S. Huang Regulation of oleosin expression in developing peanut (Arachis hypogaea L.) embryos through nucleosome loss and histone modifications J. Exp. Bot., November 1, 2009; 60(15): 4371 - 4382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Berr, L. Xu, J. Gao, V. Cognat, A. Steinmetz, A. Dong, and W.-H. Shen SET DOMAIN GROUP25 Encodes a Histone Methyltransferase and Is Involved in FLOWERING LOCUS C Activation and Repression of Flowering Plant Physiology, November 1, 2009; 151(3): 1476 - 1485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Saleh, R. Alvarez-Venegas, M. Yilmaz, O. Le, G. Hou, M. Sadder, A. Al-Abdallat, Y. Xia, G. Lu, I. Ladunga, et al. The Highly Similar Arabidopsis Homologs of Trithorax ATX1 and ATX2 Encode Proteins with Divergent Biochemical Functions PLANT CELL, March 1, 2008; 20(3): 568 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pien, D. Fleury, J. S. Mylne, P. Crevillen, D. Inze, Z. Avramova, C. Dean, and U. Grossniklaus ARABIDOPSIS TRITHORAX1 Dynamically Regulates FLOWERING LOCUS C Activation via Histone 3 Lysine 4 Trimethylation PLANT CELL, March 1, 2008; 20(3): 580 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jiang, W. Yang, Y. He, and R. M. Amasino Arabidopsis Relatives of the Human Lysine-Specific Demethylase1 Repress the Expression of FWA and FLOWERING LOCUS C and Thus Promote the Floral Transition PLANT CELL, October 1, 2007; 19(10): 2975 - 2987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Saleh, A. Al-Abdallat, I. Ndamukong, R. Alvarez-Venegas, and Z. Avramova The Arabidopsis homologs of trithorax (ATX1) and enhancer of zeste (CLF) establish 'bivalent chromatin marks' at the silent AGAMOUS locus Nucleic Acids Res., September 25, 2007; 35(18): 6290 - 6296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Deng, C. Liu, Y. Pei, X. Deng, L. Niu, and X. Cao Involvement of the Histone Acetyltransferase AtHAC1 in the Regulation of Flowering Time via Repression of FLOWERING LOCUS C in Arabidopsis Plant Physiology, April 1, 2007; 143(4): 1660 - 1668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shi and R. K. Dawe Partitioning of the Maize Epigenome by the Number of Methyl Groups on Histone H3 Lysines 9 and 27 Genetics, July 1, 2006; 173(3): 1571 - 1583. [Abstract] [Full Text] [PDF]
|
|