Published online 28 September 2005
Article |
Real-time expression profiling of microRNA precursors in human cancer cell lines
1College of Pharmacy, Ohio State University Columbus, OH, USA 2Department of Surgery, University of Oklahoma Health Sciences Center Oklahoma City, OK, USA
*To whom correspondence should be addressed. Tel: +1 614 292 3456; Fax: +1 614 292 7766; Email: schmittgen.2{at}osu.edu
Received April 27, 2005. Revised August 23, 2005. Accepted September 7, 2005.
Our previous study described a real-time PCR method to quantify microRNA (miRNA) precursors using SYBR green detection [T. D. Schmittgen, J. Jiang, Q. Liu and L. Yang (2004) Nucleic Acids Res., 32, e43]. The present study adapted the assay to a 384-well format and expanded it to include primers to 222 human miRNA precursors. TaqMan minor groove binder probes were used to discriminate nearly identical members of the let-7 family of miRNA isoforms. The miRNA precursor expression was profiled in 32 human cell lines from lung, breast, colorectal, hematologic, prostate, pancreatic, and head and neck cancers. Some miRNA precursors were expressed at similar levels in many of the cell lines, while others were differentially expressed. Clustering analysis of the miRNA precursor expression data revealed that most of the cell lines clustered into their respective tissues from which each cell line was ostensibly derived. miRNA precursor expression by PCR paralleled the mature miRNA expression by northern blotting for most of the conditions studied. Our study provides PCR primer sequences to all of the known human miRNA precursors as of December 2004 and provides a database of the miRNA precursor expression in many commonly used human cancer cell lines.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. R. Epis, K. M. Giles, A. Barker, T. S. Kendrick, and P. J. Leedman miR-331-3p Regulates ERBB-2 Expression and Androgen Receptor Signaling in Prostate Cancer J. Biol. Chem., September 11, 2009; 284(37): 24696 - 24704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Park, H. Zhou, D. Elashoff, B. S. Henson, D. A. Kastratovic, E. Abemayor, and D. T. Wong Salivary microRNA: Discovery, Characterization, and Clinical Utility for Oral Cancer Detection Clin. Cancer Res., September 1, 2009; 15(17): 5473 - 5477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, R. Rathinam, A. Walch, and S. K. Alahari ST14 (Suppression of Tumorigenicity 14) Gene Is a Target for miR-27b, and the Inhibitory Effect of ST14 on Cell Growth Is Independent of miR-27b Regulation J. Biol. Chem., August 21, 2009; 284(34): 23094 - 23106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Sotiropoulou, G. Pampalakis, E. Lianidou, and Z. Mourelatos Emerging roles of microRNAs as molecular switches in the integrated circuit of the cancer cell RNA, August 1, 2009; 15(8): 1443 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Wickramasinghe, T. T. Manavalan, S. M. Dougherty, K. A. Riggs, Y. Li, and C. M. Klinge Estradiol downregulates miR-21 expression and increases miR-21 target gene expression in MCF-7 breast cancer cells Nucleic Acids Res., May 1, 2009; 37(8): 2584 - 2595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S M Khoshnaw, A R Green, D G Powe, and I O Ellis MicroRNA involvement in the pathogenesis and management of breast cancer J. Clin. Pathol., May 1, 2009; 62(5): 422 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lebanony, H. Benjamin, S. Gilad, M. Ezagouri, A. Dov, K. Ashkenazi, N. Gefen, S. Izraeli, G. Rechavi, H. Pass, et al. Diagnostic Assay Based on hsa-miR-205 Expression Distinguishes Squamous From Nonsquamous Non-Small-Cell Lung Carcinoma J. Clin. Oncol., April 20, 2009; 27(12): 2030 - 2037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Nagaraj Evolving 'omics' technologies for diagnostics of head and neck cancer Brief Funct Genomic Proteomic, March 9, 2009; (2009) elp004v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Childs, M. Fazzari, G. Kung, N. Kawachi, M. Brandwein-Gensler, M. McLemore, Q. Chen, R. D. Burk, R. V. Smith, M. B. Prystowsky, et al. Low-Level Expression of MicroRNAs let-7d and miR-205 Are Prognostic Markers of Head and Neck Squamous Cell Carcinoma Am. J. Pathol., March 1, 2009; 174(3): 736 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yu, D. G. Ryan, S. Getsios, M. Oliveira-Fernandes, A. Fatima, and R. M. Lavker MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia PNAS, December 9, 2008; 105(49): 19300 - 19305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-W. Chang, C.-J. Liu, T.-H. Chu, H.-W. Cheng, P.-S. Hung, W.-Y. Hu, and S.-C. Lin Association between High miR-211 microRNA Expression and the Poor Prognosis of Oral Carcinoma Journal of Dental Research, November 1, 2008; 87(11): 1063 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ho, K. H. Ng, S. Rosen, A. Dostal, R. I. Gregory, and J. A. Kreidberg Podocyte-Specific Loss of Functional MicroRNAs Leads to Rapid Glomerular and Tubular Injury J. Am. Soc. Nephrol., November 1, 2008; 19(11): 2069 - 2075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. El Ouaamari, N. Baroukh, G. A. Martens, P. Lebrun, D. Pipeleers, and E. van Obberghen miR-375 Targets 3'-Phosphoinositide-Dependent Protein Kinase-1 and Regulates Glucose-Induced Biological Responses in Pancreatic {beta}-Cells Diabetes, October 1, 2008; 57(10): 2708 - 2717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Markou, E. G. Tsaroucha, L. Kaklamanis, M. Fotinou, V. Georgoulias, and E. S. Lianidou Prognostic Value of Mature MicroRNA-21 and MicroRNA-205 Overexpression in Non-Small Cell Lung Cancer by Quantitative Real-Time RT-PCR Clin. Chem., October 1, 2008; 54(10): 1696 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, J. Lozach, E. W. Garcia, B. Barnes, S. Luo, I. Mikoulitch, L. Zhou, G. Schroth, and J.-B. Fan Highly sensitive and specific microRNA expression profiling using BeadArray technology Nucleic Acids Res., August 1, 2008; 36(14): e87 - e87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kefas, J. Godlewski, L. Comeau, Y. Li, R. Abounader, M. Hawkinson, J. Lee, H. Fine, E. A. Chiocca, S. Lawler, et al. microRNA-7 Inhibits the Epidermal Growth Factor Receptor and the Akt Pathway and Is Down-regulated in Glioblastoma Cancer Res., May 15, 2008; 68(10): 3566 - 3572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-W. Lee, C. H. Choi, J.-J. Choi, Y.-A. Park, S.-J. Kim, S. Y. Hwang, W. Y. Kim, T.-J. Kim, J.-H. Lee, B.-G. Kim, et al. Altered MicroRNA Expression in Cervical Carcinomas Clin. Cancer Res., May 1, 2008; 14(9): 2535 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Davis, T. L. Cuellar, S. M. Koch, A. J. Barker, B. D. Harfe, M. T. McManus, and E. M. Ullian Conditional Loss of Dicer Disrupts Cellular and Tissue Morphogenesis in the Cortex and Hippocampus J. Neurosci., April 23, 2008; 28(17): 4322 - 4330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. O'Hara, W. Vahrson, and D. P. Dittmer Gene alteration and precursor and mature microRNA transcription changes contribute to the miRNA signature of primary effusion lymphoma Blood, February 15, 2008; 111(4): 2347 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jiang, Y. Gusev, I. Aderca, T. A. Mettler, D. M. Nagorney, D. J. Brackett, L. R. Roberts, and T. D. Schmittgen Association of MicroRNA Expression in Hepatocellular Carcinomas with Hepatitis Infection, Cirrhosis, and Patient Survival Clin. Cancer Res., January 15, 2008; 14(2): 419 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Lee, M. Baek, Y. Gusev, D. J. Brackett, G. J. Nuovo, and T. D. Schmittgen Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors RNA, January 1, 2008; 14(1): 35 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Lee, Z. Deng, C.-H. Wang, and B. B. Yang MicroRNA-378 promotes cell survival, tumor growth, and angiogenesis by targeting SuFu and Fus-1 expression PNAS, December 18, 2007; 104(51): 20350 - 20355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-B. Shi, L. Xue, J. Yang, A.-H. Ma, J. Zhao, M. Xu, C. G. Tepper, C. P. Evans, H.-J. Kung, and R. W. deVere White An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent growth of prostate cancer cells PNAS, December 11, 2007; 104(50): 19983 - 19988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Tang, J. Gal, X. Zhuang, W. Wang, H. Zhu, and G. Tang A simple array platform for microRNA analysis and its application in mouse tissues RNA, October 1, 2007; 13(10): 1803 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sathyan, H. B. Golden, and R. C. Miranda Competing Interactions between Micro-RNAs Determine Neural Progenitor Survival and Proliferation after Ethanol Exposure: Evidence from an Ex Vivo Model of the Fetal Cerebral Cortical Neuroepithelium J. Neurosci., August 8, 2007; 27(32): 8546 - 8557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Maroney, S. Chamnongpol, F. Souret, and T. W. Nilsen A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation RNA, June 1, 2007; 13(6): 930 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Beuvink, F. A. Kolb, W. Budach, A. Garnier, J. Lange, F. Natt, U. Dengler, J. Hall, W. Filipowicz, and J. Weiler A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs Nucleic Acids Res., April 1, 2007; 35(7): e52 - e52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Ritland Politz, F. Zhang, and T. Pederson MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cells PNAS, December 12, 2006; 103(50): 18957 - 18962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ason, D. K. Darnell, B. Wittbrodt, E. Berezikov, W. P. Kloosterman, J. Wittbrodt, P. B. Antin, and R. H. A. Plasterk From the Cover: Differences in vertebrate microRNA expression PNAS, September 26, 2006; 103(39): 14385 - 14389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Weber, R. E. Teresi, C. E. Broelsch, A. Frilling, and C. Eng A Limited Set of Human MicroRNA Is Deregulated in Follicular Thyroid Carcinoma J. Clin. Endocrinol. Metab., September 1, 2006; 91(9): 3584 - 3591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Castoldi, S. Schmidt, V. Benes, M. Noerholm, A. E. Kulozik, M. W. Hentze, and M. U. Muckenthaler A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA) RNA, May 1, 2006; 12(5): 913 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Mattick and I. V. Makunin Non-coding RNA. Hum. Mol. Genet., April 15, 2006; 15(suppl_1): R17 - R29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Tang, P. Hajkova, S. C. Barton, K. Lao, and M. A. Surani MicroRNA expression profiling of single whole embryonic stem cells Nucleic Acids Res., January 24, 2006; 34(2): e9 - e9. [Abstract] [Full Text] [PDF]
|
|