Skip Navigation

Nucleic Acids Research 2005 33(18):5851-5860; doi:10.1093/nar/gki898
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (980K) Freely available
Right arrow Screen PDF (316K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (41)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vorlícková, M.
Right arrow Articles by Kypr, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vorlícková, M.
Right arrow Articles by Kypr, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 12 October 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats

Michaela Vorlícková*, Jana Chládková, Iva Kejnovská, Markéta Fialová and Jaroslav Kypr

Institute of Biophysics, Academy of Sciences of the Czech Republic Královopolská 135, CZ-612 65 Brno, Czech Republic

*To whom correspondence should be addressed. Tel: +42 0 541 517 188; Fax: +42 0 541 240 497; Email: mifi{at}ibp.cz

Received July 11, 2005. Revised September 12, 2005. Accepted September 28, 2005.

Secondary structures of the G-rich strand of human telomere DNA fragments G3(TTAG3)n, n = 1–16, have been studied by means of circular dichroism spectroscopy and PAGE, in solutions of physiological potassium cation concentrations. It has been found that folding of these fragments into tetraplexes as well as tetraplex thermostabilities and enthalpy values depend on the number of TTAG3 repeats. The suggested topologies include, e.g. antiparallel and parallel bimolecular tetraplexes, an intramolecular antiparallel tetraplex, a tetraplex consisting of three parallel chains and one antiparallel chain, a poorly stable parallel intramolecular tetraplex, and both parallel and antiparallel tetramolecular tetraplexes. G3(TTAG3)3 folds into a single, stable and very compact intramolecular antiparallel tetraplex. With an increasing repeat number, the fragment tetraplexes surprisingly are ever less thermostable and their migration and enthalpy decrease indicate increasing irregularities or domain splitting in their arrangements. Reduced stability and different topology of lengthy telomeric tails could contribute to the stepwise telomere shortening process.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. I. Onyshchenko, T. I. Gaynutdinov, E. A. Englund, D. H. Appella, R. D. Neumann, and I. G. Panyutin
Stabilization of G-quadruplex in the BCL2 promoter region in double-stranded DNA by invading short PNAs
Nucleic Acids Res., October 9, 2009; (2009) gkp840v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. W. Lim, P. Alberti, A. Guedin, L. Lacroix, J.-F. Riou, N. J. Royle, J.-L. Mergny, and A. T. Phan
Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G{middle dot}C{middle dot}G{middle dot}C tetrad
Nucleic Acids Res., October 1, 2009; 37(18): 6239 - 6248.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Renciuk, I. Kejnovska, P. Skolakova, K. Bednarova, J. Motlova, and M. Vorlickova
Arrangements of human telomere DNA quadruplex in physiologically relevant K+ solutions
Nucleic Acids Res., October 1, 2009; 37(19): 6625 - 6634.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. D. Schonhoft, R. Bajracharya, S. Dhakal, Z. Yu, H. Mao, and S. Basu
Direct experimental evidence for quadruplex-quadruplex interaction within the human ILPR
Nucleic Acids Res., June 1, 2009; 37(10): 3310 - 3320.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. M. Pedroso, W. Hayward, and T. M. Fletcher
The effect of the TRF2 N-terminal and TRFH regions on telomeric G-quadruplex structures
Nucleic Acids Res., April 1, 2009; 37(5): 1541 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Kypr, I. Kejnovska, D. Renciuk, and M. Vorlickova
Circular dichroism and conformational polymorphism of DNA
Nucleic Acids Res., April 1, 2009; 37(6): 1713 - 1725.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Tsumagari, L. Qi, K. Jackson, C. Shao, M. Lacey, J. Sowden, R. Tawil, V. Vedanarayanan, and M. Ehrlich
Epigenetics of a tandem DNA repeat: chromatin DNaseI sensitivity and opposite methylation changes in cancers
Nucleic Acids Res., April 1, 2008; 36(7): 2196 - 2207.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Tang, Z.-y. Kan, Y. Yao, Q. Wang, Y.-h. Hao, and Z. Tan
G-quadruplex preferentially forms at the very 3' end of vertebrate telomeric DNA
Nucleic Acids Res., March 27, 2008; 36(4): 1200 - 1208.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. J. Patel, A. T. Phan, and V. Kuryavyi
Human telomere, oncogenic promoter and 5'-UTR G-quadruplexes: diverse higher order DNA and RNA targets for cancer therapeutics
Nucleic Acids Res., December 3, 2007; 35(22): 7429 - 7455.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. T. Phan, V. Kuryavyi, K. N. Luu, and D. J. Patel
Structure of two intramolecular G-quadruplexes formed by natural human telomere sequences in K+ solution
Nucleic Acids Res., October 8, 2007; 35(19): 6517 - 6525.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Saydam, R. Kanagaraj, T. Dietschy, P. L. Garcia, J. Pena-Diaz, I. Shevelev, I. Stagljar, and P. Janscak
Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors
Nucleic Acids Res., September 27, 2007; 35(17): 5706 - 5716.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Dai, M. Carver, C. Punchihewa, R. A. Jones, and D. Yang
Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence
Nucleic Acids Res., August 1, 2007; 35(15): 4927 - 4940.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C.-C. Chang, C.-W. Chien, Y.-H. Lin, C.-C. Kang, and T.-C. Chang
Investigation of spectral conversion of d(TTAGGG)4 and d(TTAGGG)13 upon potassium titration by a G-quadruplex recognizer BMVC molecule
Nucleic Acids Res., May 14, 2007; 35(9): 2846 - 2860.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
H. Torigoe and A. Furukawa
Tetraplex Structure of Fission Yeast Telomeric DNA and Unfolding of the Tetraplex on the Interaction with Telomeric DNA Binding Protein Pot1
J. Biochem., January 1, 2007; 141(1): 57 - 68.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. T. Phan, K. N. Luu, and D. J. Patel
Different loop arrangements of intramolecular human telomeric (3+1) G-quadruplexes in K+ solution
Nucleic Acids Res., November 14, 2006; 34(19): 5715 - 5719.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. R. Salas, I. Petruseva, O. Lavrik, A. Bourdoncle, J.-L. Mergny, A. Favre, and C. Saintome
Human replication protein A unfolds telomeric G-quadruplexes
Nucleic Acids Res., October 18, 2006; 34(17): 4857 - 4865.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Cogoi and L. E. Xodo
G-quadruplex formation within the promoter of the KRAS proto-oncogene and its effect on transcription.
Nucleic Acids Res., January 1, 2006; 34(9): 2536 - 2549.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.