Skip Navigation

Nucleic Acids Research 2005 33(2):786-795; doi:10.1093/nar/gki228
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1974K) Freely available
Right arrow Screen PDF (579K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Takiya, S.
Right arrow Articles by Suzuki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takiya, S.
Right arrow Articles by Suzuki, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 1 February 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Fibroin-modulator-binding protein-1 (FMBP-1) contains a novel DNA-binding domain, repeats of the score and three amino acid peptide (STP), conserved from Caenorhabditis elegans to humans

Shigeharu Takiya1,2,*, Tetsurou Ishikawa2, Katsuya Ohtsuka2, Yoshinori Nishita1 and Yoshiaki Suzuki3

1 Center for Advanced Science and Technology and Hokkaido University North 10, West 8, Kita-ku, Sapporo 060-0810, Japan 2 Graduate School of Environmental Earth Science, Hokkaido University North 10, West 8, Kita-ku, Sapporo 060-0810, Japan 3 National Institute for Basic Biology Nishigo-naka 38, Okazaki 444-8585, Japan

*To whom correspondence should be addressed. Tel: +81 11 706 3590; Fax: +81 11 737 0536; Email: takiya{at}rmg.hokudai.ac.jp

Received October 14, 2004. Revised January 18, 2004. Accepted January 18, 2004.

The predicted transcriptional regulatory factor for the fibroin gene of the silkworm Bombyx mori, fibroin-modulator-binding protein-1 (FMBP-1), was purified by sequential DNA affinity column chromatography, and cDNA clones corresponding to FMBP-1 were isolated from a library. The N-terminal half of FMBP-1 has a weak similarity to the DNA-binding domain of several transcriptional regulatory factors in higher plants. The C-terminal half contains four tandem repeats of a novel 23 amino acid motif, which we named the score and three amino acid peptide (STP). Other genes containing STP repeats were found in Drosophila, Caenorhabditis elegans, mouse and human. Mutational analysis of FMBP-1 showed that the STP repeats form a novel DNA-binding domain. Sequences flanking STP repeats modulated DNA-binding activity. The FMBP-1 gene was expressed during the fourth to fifth instar. FMBP-1 activity appeared to be regulated at the transcriptional level and by the post-transcriptional modification.

Present address: Yoshiaki Suzuki, 5651-451 Matsukawa Village, North Azumi County, Nagano Prefecture 399-8501, Japan

DDBJ/EMBL/GenBank accession no. AB163434


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J BiochemHome page
S. Takiya, S. Saito, T. Yokoyama, D. Matsumoto, T. Aizawa, M. Kamiya, M. Demura, and K. Kawano
DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori
J. Biochem., July 1, 2009; 146(1): 103 - 111.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.