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Nucleic Acids Research 2005 33(20):6555-6565; doi:10.1093/nar/gki964
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Published online 24 November 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex

Chunyu Han, Hao Liu1, Jin Liu, Kang Yin2, Yi Xie2, Xiao Shen, Yong Wang, Jiangang Yuan, Boqing Qiang, Yong-Jian Liu1,* and Xiaozhong Peng

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Chinese National Human Genome Center Beijing, People's Republic of China 1Departments of Neurology and Neurobiology, University of Pittsburgh School of Medicine Pittsburgh, PA 15213, USA 2College of Biology, Fudan University Shanghai, People's Republic of China

*To whom correspondence should be addressed. Tel: +1 412648 3730; Fax: +1 412 624 9914; Email: yjliu{at}pitt.edu

Received September 20, 2005. Revised October 31, 2005. Accepted October 31, 2005.

Human Bex2 (brain expressed X-linked, hBex2) is highly expressed in the embryonic brain, but its function remains unknown. We have identified that LMO2, a LIM-domain containing transcriptional factor, specifically interacts with hBex2 but not with mouse Bex1 and Bex2. The interaction was confirmed both by pull-down with GST-hBex2 and by coimmunoprecipitation assays in vivo. Using electrophoretic mobility shift assay, we have demonstrated the physical interaction of hBex2 and LMO2 as part of a DNA-binding protein complex. We have also shown that hBex2 can enhance the transcriptional activity of LMO2 in vivo. Furthermore, using mammalian two-hybrid analysis, we have identified a neuronal bHLH protein, NSCL2, as a novel binding partner for LMO2. We then showed that LMO2 could up-regulate NSCL2-dependent transcriptional activity, and hBex2 augmented this effect. Thus, hBex2 may act as a specific regulator during embryonic development by modulating the transcriptional activity of a novel E-box sequence-binding complex that contains hBex2, LMO2, NSCL2 and LDB1.

or Xiaozhong Peng. Tel: +86 10 65296411; Fax: +86 10 65240529; Email: pengxiaozhong{at}pumc.edu.cn


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