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Nucleic Acids Research 2005 33(21):6795-6804; doi:10.1093/nar/gki982
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Published online 7 December 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

MPP6 is an exosome-associated RNA-binding protein involved in 5.8S rRNA maturation

Geurt Schilders1, Reinout Raijmakers1, Jos M. H. Raats1,2 and Ger J. M. Pruijn1,*

1Department of Biochemistry, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Nijmegen, The Netherlands 2ModiQuest B.V. Nijmegen, The Netherlands

*To whom correspondence should be addressed. Tel: +31 24 361 6847; Fax: +31 24 354 0525; Email: G.Pruijn{at}ncmls.ru.nl

Received October 20, 2005. Revised November 9, 2005. Accepted November 9, 2005.

The exosome is a complex of 3'->5' exoribonucleases which is involved in many RNA metabolic processes. To regulate these functions distinct proteins are believed to recruit the exosome to specific substrate RNAs. Here, we demonstrate that M-phase phosphoprotein 6 (MPP6), a protein reported previously to co-purify with the TAP-tagged human exosome, accumulates in the nucleoli of HEp-2 cells and associates with a subset of nuclear exosomes as evidenced by co-immunoprecipitation and biochemical fractionation experiments. In agreement with its nucleolar accumulation, siRNA-mediated knock-down experiments revealed that MPP6 is involved in the generation of the 3' end of the 5.8S rRNA. The accumulation of the same processing intermediates after reducing the levels of either MPP6 or exosome components strongly suggests that MPP6 is required for the recruitment of the exosome to the pre-rRNA. Interestingly, MPP6 appeared to display RNA-binding activity in vitro with a preference for pyrimidine-rich sequences, and to bind to the ITS2 element of pre-rRNAs. Our data indicate that MPP6 is a nucleolus-specific exosome co-factor required for its role in the maturation of 5.8S rRNA.


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