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Nucleic Acids Research 2005 33(21):6942-6951; doi:10.1093/nar/gki998
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Published online 6 December 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
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Article

Transcription regulation of the EcoRV restriction–modification system

Ekaterina Semenova1, Leonid Minakhin1, Ekaterina Bogdanova3, Maxim Nagornykh3, Anatoliy Vasilov1, Tomasz Heyduk4, Alexander Solonin3, Marina Zakharova3 and Konstantin Severinov1,2,5,*

1Waksman Institute for Microbiology, Rutgers, the State University of New Jersey Piscataway, NJ 08854, USA 2Department of Biochemistry and Molecular Biology, Rutgers, the State University of New Jersey Piscataway, NJ 08854, USA 3Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences Puschino 142292, Russia 4E. A. Doisy Department of Biochemistry and Molecular Biology, St Louis University Medical School St Louis, MO 63104, USA 5Institute of Molecular Genetics Moscow, Russia

*To whom correspondence should be addressed at Waksman Institute for Microbiology, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA. Tel: +1 732 445 6095; Fax: +1 732 445 5735; Email: severik{at}waksman.rutgers.edu

Received October 17, 2005. Revised November 16, 2005. Accepted November 16, 2005.

When a plasmid containing restriction–modification (R–M) genes enters a naïve host, unmodified host DNA can be destroyed by restriction endonuclease. Therefore, expression of R–M genes must be regulated to ensure that enough methyltransferase is produced and that host DNA is methylated before the endonuclease synthesis begins. In several R–M systems, specialized Control (C) proteins coordinate expression of the R and the M genes. C proteins bind to DNA sequences called C-boxes and activate expression of their cognate R genes and inhibit the M gene expression, however the mechanisms remain undefined. Here, we studied the regulation of gene expression in the C protein-dependent EcoRV system. We map the divergent EcoRV M and R gene promoters and we define the site of C protein-binding that is sufficient for activation of the EcoRV R transcription.

Correspondence may also be addressed to Marina Zakharova. Tel: +7 096 773 2598; Fax: +7 095 956 3370; Email: zemskovam{at}mail.ru

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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