Published online 10 May 2005
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An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo São Paulo, SP 05508-900, Brazil
*To whom correspondence should be addressed at Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Avenue Professor Lineu Prestes, 1374, São Paulo, SP 05508-900, Brazil. Tel: +55 11 3091 7499; Fax: +55 11 3091 7354; Email: cfmmenck{at}usp.br
Received February 25, 2005. Revised April 15, 2005. Accepted April 15, 2005.
DNA polymerases of the Y-family, such as Escherichia coli UmuC and DinB, are specialized enzymes induced by the SOS response, which bypass lesions allowing the continuation of DNA replication. umuDC orthologs are absent in Caulobacter crescentus and other bacteria, raising the question about the existence of SOS mutagenesis in these organisms. Here, we report that the C.crescentus dinB ortholog is not involved in damage-induced mutagenesis. However, an operon composed of two hypothetical genes and dnaE2, encoding a second copy of the catalytic subunit of Pol III, is damage inducible in a recA-dependent manner, and is responsible for most ultraviolet (UV) and mitomycin C-induced mutations in C.crescentus. The results demonstrate that the three genes are required for the error-prone processing of DNA lesions. The two hypothetical genes were named imuA and imuB, after inducible mutagenesis. ImuB is similar to proteins of the Y-family of polymerases, and possibly cooperates with DnaE2 in lesion bypass. The mutations arising as a consequence of the activity of the imuAB dnaE2 operon are rather unusual for UV irradiation, including G:C to C:G transversions.
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