Nucleic Acids Research, 2005, Vol. 33, Database issue D121-D124
© 2005, the authors
Nucleic Acids Research, Vol. 33, Database issue © Oxford University Press 2005; all rights reserved
Rfam: annotating non-coding RNAs in complete genomes
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK and 1 Howard Hughes Medical Institute and Department of Genetics, Washington University School of Medicine, St Louis, MO 63108, USA
* To whom correspondence should be addressed. Tel: +44 1223 834244; Fax: +44 1223 494919; Email: sgj{at}sanger.ac.uk
Received September 15, 2004; Revised and Accepted October 8, 2004
Rfam is a comprehensive collection of non-coding RNA (ncRNA) families, represented by multiple sequence alignments and profile stochastic context-free grammars. Rfam aims to facilitate the identification and classification of new members of known sequence families, and distributes annotation of ncRNAs in over 200 complete genome sequences. The data provide the first glimpses of conservation of multiple ncRNA families across a wide taxonomic range. A small number of large families are essential in all three kingdoms of life, with large numbers of smaller families specific to certain taxa. Recent improvements in the database are discussed, together with challenges for the future. Rfam is available on the Web at http://www.sanger.ac.uk/Software/Rfam/ and http://rfam.wustl.edu/.
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