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Nucleic Acids Research 2005 33(Database Issue):D633-D636; doi:10.1093/nar/gki052
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Nucleic Acids Research, 2005, Vol. 33, Database issue D633-D636
© 2005, the authors
Nucleic Acids Research, Vol. 33, Database issue © Oxford University Press 2005; all rights reserved

Arabidopsis nucleolar protein database (AtNoPDB)

John W. S. Brown*, Peter J. Shaw2, Paul Shaw1 and David F. Marshall1

Gene Expression Programme and 1 Computational Biology Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK and 2 John Innes Centre, Norwich NR4 7UH, UK

* To whom correspondence should be addressed. Tel: +44 1382 568533; Fax: +44 1382 562426; Email: jbrown{at}scri.sari.ac.uk

Received August 13, 2004; Revised and Accepted October 1, 2004

The Arabidopsis Nucleolar Protein Database (http://bioinf.scri.sari.ac.uk/cgi-bin/atnopdb/home) provides information on 217 proteins identified in a proteomic analysis of nucleoli isolated from Arabidopsis cell culture. The database is organized on the basis of the Arabidopsis gene identifier number. The information provided includes protein description, protein class, whether or not the plant protein has a homologue in the most recent human nucleolar proteome and the results of reciprocal BLAST analysis of the human proteome. In addition, for one-third of the 217 Arabidopsis nucleolar proteins, localization images are available from analysis of full-length cDNA–green fluorescent protein (GFP) fusions and the strength of signal in different parts of the cell—nucleolus, nucleolus-associated structures, nucleoplasm, nuclear bodies and extra-nuclear—is provided. For each protein, the most likely human and yeast orthologues, where identifiable through BLASTX analysis, are given with links to relevant information sources.

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