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E-RNAi: a web application to design optimized RNAi constructs
Boveri-Group Signaling and Functional Genomics, German Cancer Research Center Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
*To whom correspondence should be addressed. Tel: +49 6221 421951; Fax: +49 6221 421959; Email: m.boutros{at}dkfz.de
Received February 14, 2005. Revised April 13, 2005. Accepted April 13, 2005.
RNA interference (RNAi) has become a powerful genetic approach to systematically dissect gene function on a genome-wide scale. Owing to the penetrance and efficiency of RNAi in invertebrates, model organisms such as Drosophila melanogaster and Caenorhabditis elegans have contributed significantly to the identification of novel components of diverse biological pathways, ranging from early development to fat storage and aging. For the correct assessment of phenotypes, a key issue remains the stringent quality control of long double-stranded RNAs (dsRNA) to calculate potential off-target effects that may obscure the phenotypic data. We here describe a web-based tool to evaluate and design optimized dsRNA constructs. Moreover, the application also gives access to published predesigned dsRNAs. The E-RNAi web application is available at http://e-rnai.dkfz.de/.
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