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Nucleic Acids Research 2006 34(1):120-129; doi:10.1093/nar/gkj418
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Published online 10 January 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
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Article

Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination

Gema López-Torrejón1, María I. Martínez-Jiménez1 and Silvia Ayora1,2,*

1Department of Microbial Biotechnology, Centro Nacional Biotecnología, CSIC Darwin 3, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain 2Department of Molecular Biology, Universidad Autónoma de Madrid Darwin 2, Cantoblanco, 28049 Madrid, Spain

*To whom correspondence should be addressed. Tel: +34 91585 4528; Fax: +34 91585 4506, Email: sylvia.ayora{at}uam.es

Received October 10, 2005. Revised December 13, 2005. Accepted December 13, 2005.

Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein, which binds both single-stranded (ss) and double-stranded (ds) DNA and facilitates the formation of higher order protein–DNA complexes in vitro. LrpC binds at different sites within the same DNA molecule promoting intramolecular ligation. When bound to separate molecules, it promotes intermolecular ligation, and joint molecule formation between a circular ssDNA and a homologous ssDNA-tailed linear dsDNA. LrpC binding showed a higher affinity for 4-way (Holliday) junctions in their open conformation, when compared with curved dsDNA. Consistent with these biochemical activities, an lrpC null mutant strain rendered cells sensitive to DNA damaging agents such as methyl methanesulfonate and 4-nitroquinoline-1-oxide, and showed a segregation defect. These findings collectively suggest that LrpC may be involved in DNA transactions during DNA repair and recombination.


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