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Nucleic Acids Research 2006 34(1):232-242; doi:10.1093/nar/gkj423
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Published online 5 January 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens

Bénédicte Trouiller, Didier G. Schaefer1, Florence Charlot and Fabien Nogué*

Station de Génétique et d'Amélioration des Plantes, INRA Route de St Cyr, 78026 Versailles, France 1Département de biologie moléculaire végétale, Université de Lausanne CH-1015 Lausanne, Switzerland

*To whom correspondence should be addressed. Tel: +33 1 30833009; Fax: +33 1 30833319; Email: nogue{at}versailles.inra.fr

Received November 18, 2005. Accepted December 14, 2005.

MSH2 is a central component of the mismatch repair pathway that targets mismatches arising during DNA replication, homologous recombination (HR) and in response to genotoxic stresses. Here, we describe the function of MSH2 in the moss Physcomitrella patens, as deciphered by the analysis of loss of function mutants. Ppmsh2 mutants display pleiotropic growth and developmental defects, which reflect genomic instability. Based on loss of function of the APT gene, we estimated this mutator phenotype to be at least 130 times higher in the mutants than in wild type. We also found that MSH2 is involved in some but not all the moss responses to genotoxic stresses we tested. Indeed, the Ppmsh2 mutants were more tolerant to cisplatin and show higher sensitivity to UV-B radiations. PpMSH2 gene involvement in HR was studied by assessing gene targeting (GT) efficiency with homologous and homeologous sequences. GT efficiency with homologous sequences was slightly decreased in the Ppmsh2 mutant compared with wild type. Strikingly GT efficiency with homeologous sequences decreased proportionally to sequence divergence in the wild type whereas it remained unaffected in the mutants. Those results demonstrate the role of PpMSH2 in the maintenance of genome integrity and in homologous and homeologous recombination.

Present address: Didier Schaefer, Station de Génétique et d'Amélioration des Plantes, INRA, Route de St Cyr, 78026 Versailles, France


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