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Nucleic Acids Research 2006 34(1):243-253; doi:10.1093/nar/gkj425
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Published online 9 January 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

Brain-specific promoter and polyadenylation sites of the ß-adducin pre-mRNA generate an unusually long 3'-UTR

Luisa Costessi, Giulia Devescovi, Francisco E. Baralle and Andrés F. Muro*

International Centre for Genetic Engineering and Biotechnology Trieste, Italy

*To whom correspondence should be addressed. Tel: +39 040 3757312; Fax: +39 040 226555; Email: muro{at}icgeb.org

Received November 11, 2005. Revised December 15, 2005. Accepted December 15, 2005.

Adducins are a family of membrane skeleton proteins composed of {alpha}-, ß- and {gamma}-subunits that promote actin and spectrin association in erythrocytes. The {alpha}- and {gamma}-subunits are expressed ubiquitously, while the ß-subunit is found in brain and erythropoietic tissues. The brain ß-adducin protein is similar in size to that of spleen, but the mRNA transcript is a brain-specific one that has not been yet characterized, having an estimated length of 8–9 kb instead of the 3–4 kb of spleen mRNA. Here, we show the molecular basis for these differences by determining the structure of the brain-specific ß-adducin transcript in rats, mice and humans. We identified a brain-specific promoter in rodents that, apparently, was not conserved in humans. In addition, we present evidence that the brain-mRNAs are formed by a common mechanism consisting in the tissue-specific use of alternative polyadenylation sites generating unusually long 3'-untranslated region of up to 6.6 kb. This hypothesis is supported by the presence of highly-conserved regions flanking the brain-specific polyadenylation site that suggest the involvement of these sequences in the translational regulation, stability and/or subcellular localization of the ß-adducin transcript in the brain.

DDBJ/EMBL/GenBank accession no. DQ231568


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