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Nucleic Acids Research 2006 34(1):e2; doi:10.1093/nar/gnj004
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Published online 5 January 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Methods Online

Fast chromatin immunoprecipitation assay

Joel D. Nelson1,2, Oleg Denisenko2, Pavel Sova2 and Karol Bomsztyk1,2,*

1Molecular and Cellular Biology Program, University of Washington Seattle, WA 98109, USA 2UW Medicine Lake Union Research, University of Washington Seattle, WA 98109, USA

*To whom correspondence should be addressed at UW Medicine Lake Union, Box 358050, University of Washington, Seattle, WA 98109, USA. Tel: +1 206 616 7949; Fax: +1 206 616 8591; Email: karolb{at}u.washington.edu

Received November 15, 2005. Revised December 12, 2005. Accepted December 12, 2005.

Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol–chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.


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