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Nucleic Acids Research 2006 34(10):2878-2886; doi:10.1093/nar/gkl081
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Published online 31 May 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Article

The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif

Eveline Lescrinier, Koen Nauwelaerts, Katia Zanier1, Koen Poesen1, Michael Sattler1 and Piet Herdewijn*

Laboratory of Medicinal Chemistry, Rega Institute for Medical Research BioMacs, K.U.Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium 1 EMBL, Structural & Computational Biology and Gene Expression Meyerhofstrasse 1, D-69117 Heidelberg, Germany

*To whom correspondence should be addressed. Tel: +32 0 16 337387; Fax: +32 0 16 337340; Email: piet.herdewijn{at}rega.kuleuvan.ac.be

Received December 23, 2005. Revised January 23, 2006. Accepted March 6, 2006.

Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem–loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem–loop of lys-tRNAUUU. A comparison of this stem–loop with our structure demonstrates different effects of the modification depending on the loop sequence.


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