Skip Navigation

Nucleic Acids Research 2006 34(13):3803-3810; doi:10.1093/nar/gkl532
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (131K) Freely available
Right arrow Screen PDF (132K) Freely available
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Strat, A.
Right arrow Articles by Giordano, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strat, A.
Right arrow Articles by Giordano, T.
Related Collections
Right arrow Targeted inhibition of gene function
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 11 August 2006

Nucleic Acids Research, 2006, Vol. 34, No. 13 3803-3810
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article

Specific and nontoxic silencing in mammalian cells with expressed long dsRNAs

Aurel Strat1, Lu Gao1, Tada Utsuki1, Bing Cheng1, Sam Nuthalapaty1, J. Mike Mathis2, Yoshinobu Odaka2 and Tony Giordano1,3,*

1 Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center Shreveport, LA, USA 2 Department of Cell Biology and Anatomy, Louisiana State University Health Sciences Center Shreveport, LA, USA 3 Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center Shreveport, LA, USA

*To whom correspondence should be addressed. Tel: +1 318 675 7791; Fax: +1 318 675 5180; Email: agiord{at}lsuhsc.edu

Received March 8, 2006. Revised May 27, 2006. Accepted July 10, 2006.

A number of groups have developed libraries of siRNAs to identify genes through functional genomics. While these studies have validated the approach of making functional RNAi libraries to understand fundamental cellular mechanisms, they require information and knowledge of existing sequences since the RNAi sequences are generated synthetically. An alternative strategy would be to create an RNAi library from cDNA. Unfortunately, the complexity of such a library of siRNAs would make screening difficult. To reduce the complexity, longer dsRNAs could be used; however, concerns of induction of the interferon response and off-target effects of long dsRNAs have prevented their use. As a first step in creating such libraries, long dsRNA was expressed in mammalian cells. The 250 nt dsRNAs were capable of efficiently silencing a luciferase reporter gene that was stably transfected in MDA-MB-231 cells without inducing the interferon response or off-target effects any more than reported for siRNAs. In addition, a long dsRNA expressed in the same cell line was capable of silencing endogenous c-met expression and inhibited cell migration, whereas the dsRNA against luciferase had no effect on c-met or cell migration. The studies suggest that large dsRNA libraries are feasible and that functional selection of genes will be possible.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
S. Dedieu, B. Langlois, J. Devy, B. Sid, P. Henriet, H. Sartelet, G. Bellon, H. Emonard, and L. Martiny
LRP-1 Silencing Prevents Malignant Cell Invasion despite Increased Pericellular Proteolytic Activities
Mol. Cell. Biol., May 1, 2008; 28(9): 2980 - 2995.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. P. Aalto, L. P. Sarin, A. A. van Dijk, M. Saarma, M. M. Poranen, U. Arumae, and D. H. Bamford
Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage {phi}6 RNA-dependent RNA polymerase
RNA, March 1, 2007; 13(3): 422 - 429.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.