Nucleic Acids Research Advance Access originally published online on August 25, 2006
Nucleic Acids Research 2006 34(15):4189-4197; doi:10.1093/nar/gkl500
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Nucleic Acids Research, 2006, Vol. 34, No. 15 4189-4197
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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DEAD-box RNA helicases in Escherichia coli
Laboratoire de Génétique Moléculaire, CNRS UMR8541 Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France
*To whom correspondence should be addressed. Tel: +33 1 44 32 35 41; Fax: +33 1 44 32 39 41; Email: iost{at}biologie.ens.fr
*Correspondence may also be addressed to Marc Dreyfus. Tel: +33 1 44 32 35 26; Fax: +33 1 44 32 39 41; Email: mdreyfus{at}biologie.ens.fr
Received March 31, 2006. Revised June 29, 2006. Accepted June 30, 2006.
In spite of their importance in RNA metabolism, the function of DExD/H-box proteins (including DEAD-box proteins) is poorly understood at the molecular level. Here, we present recent progress achieved with the five DEAD-box proteins from Escherichia coli, which have been particularly well studied. These proteins, which have orthologues in many bacteria, participate, in particular, in specific steps of mRNA decay and ribosome assembly. In vitro, they behave as poorly processive RNA helicases, presumably because they only unwind a few base pairs at each cycle so that stable duplexes can reanneal rather than dissociate. Except for one of them (DbpA), these proteins lack RNA specificity in vitro, and specificity in vivo is likely conferred by partners that target them to defined substrates. Interestingly, at least one of them is multifunctional, presumably because it can interact with different partners. Altogether, several aspects of the information gathered with these proteins have become paradigms for our understanding of DEAD-box proteins in general.
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