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Nucleic Acids Research Advance Access originally published online on August 26, 2006
Nucleic Acids Research 2006 34(16):4387-4394; doi:10.1093/nar/gkl569
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Nucleic Acids Research, 2006, Vol. 34, No. 16 4387-4394
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Nucleic Acid Enzymes

Direct and random routing of a molecular motor protein at a DNA junction

Louise K. Stanley and Mark D. Szczelkun*

DNA–Protein Interactions Unit, Department of Biochemistry, University of Bristol Bristol, BS8 1TD, UK

*To whom correspondence should be addressed. Tel: +44 117 928 7439; Fax: +44 117 928 8274; Email: mark.szczelkun{at}bristol.ac.uk

Received June 12, 2006. Revised July 10, 2006. Accepted July 21, 2006.

With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3'–5' double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks.


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