Nucleic Acids Research Advance Access originally published online on August 26, 2006
Nucleic Acids Research 2006 34(16):4395-4405; doi:10.1093/nar/gkl570
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Nucleic Acids Research, 2006, Vol. 34, No. 16 4395-4405
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Molecular Biology |
Evolutionary conservation and regulation of particular alternative splicing events in plant SR proteins
Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna Dr Bohr-Gasse 9/3, A-1030 Vienna, Austria
*To whom correspondence should be addressed. Tel: +43 1 4277 61640; Fax: +43 1 4277 9616; Email: andrea.barta{at}meduniwien.ac.at
*Correspondence may also be addressed to Maria Kalyna. Tel: +43 1 4277 61642; Fax: +43 1 4277 9616; Email: mariya.kalyna{at}univie.ac.at
Received May 9, 2006. Revised July 13, 2006. Accepted July 20, 2006.
Alternative splicing is an important mechanism for fine tuning of gene expression at the post-transcriptional level. SR proteins govern splice site selection and spliceosome assembly. The Arabidopsis genome encodes 19 SR proteins, several of which have no orthologues in metazoan. Three of the plant specific subfamilies are characterized by the presence of a relatively long alternatively spliced intron located in their first RNA recognition motif, which potentially results in an extremely truncated protein. In atRSZ33, a member of the RS2Z subfamily, this alternative splicing event was shown to be autoregulated. Here we show that atRSp31, a member of the RS subfamily, does not autoregulate alternative splicing of its similarily positioned intron. Interestingly, this alternative splicing event is regulated by atRSZ33. We demonstrate that the positions of these long introns and their capability for alternative splicing are conserved from green algae to flowering plants. Moreover, in particular alternative splicing events the splicing signals are embedded into highly conserved sequences. In different taxa, these conserved sequences occur in at least one gene within a subfamily. The evolutionary preservation of alternative splice forms together with highly conserved intron features argues for additional functions hidden in the genes of these plant-specific SR proteins.
Present addresses: Sergiy Lopato, Australian Center for Plant Functional Genomics, The University of Adelaide, PMB 1 Glen Osmond, South Australia, 5064, Australia
Viktor Voronin, Gregor Mendel-Institut, Dr Bohr-Gasse 3, A-1030 Vienna, Austria
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