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Nucleic Acids Research Advance Access originally published online on August 31, 2006
Nucleic Acids Research 2006 34(16):4429-4437; doi:10.1093/nar/gkl562
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Nucleic Acids Research, 2006, Vol. 34, No. 16 4429-4437
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

Naoyuki Sarai1,2, Wataru Kagawa1, Takashi Kinebuchi1, Ako Kagawa1, Kozo Tanaka3, Kiyoshi Miyagawa4, Shukuko Ikawa5, Takehiko Shibata5, Hitoshi Kurumizaka1,6,* and Shigeyuki Yokoyama1,2,7,*

1 RIKEN Genomic Sciences Center 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan 3 School of Life Sciences, University of Dundee, Wellcome Trust Biocentre Dundee DD1 5EH, UK 4 Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan 5 RIKEN Discovery Research Institute, Wako-shi Saitama 351-0198, Japan 6 Graduate School of Science and Engineering, Waseda University 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan 7 RIKEN Harima Institute at SPring-8, 1-1-1 Kohto Mikazuki-cho, Sayo, Hyogo 679-5148, Japan

*To whom correspondence should be addressed. Tel: +81 3 5286 8189; Fax: +81 3 5292 9211; Email: kurumizaka{at}waseda.jp

*Correspondence may also be addressed to Shigeyuki Yokoyama. Tel: +81 3 5841 4413; Fax: +81 3 5841 8057; Email: yokoyama{at}biochem.s.u-tokyo.ac.jp

Received May 2, 2006. Revised July 18, 2006. Accepted July 19, 2006.

The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1–single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.


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