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Nucleic Acids Research Advance Access originally published online on September 19, 2006
Nucleic Acids Research 2006 34(17):4996-5006; doi:10.1093/nar/gkl493
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Nucleic Acids Research, 2006, Vol. 34, No. 17 4996-5006
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

CTD kinase I is required for the integrity of the rDNA tandem array

Sabrina Grenetier, Céline Bouchoux and Valérie Goguel*

Service de Biochimie et de Génétique Moléculaire, CEA/Saclay 91191 Gif/Yvette, France

*To whom correspondence should be addressed. Tel: +33 1 4079 5123; Fax: +33 1 4079 5229; Email: valerie.goguel{at}espci.fr

Received March 23, 2006. Revised June 9, 2006. Accepted June 27, 2006.

The genomic stability of the rDNA tandem array is tightly controlled to allow sequence homogenization and to prevent deleterious rearrangements. In this report, we show that the absence of the yeast CTD kinase I (CTDK-I) complex in null mutant strains leads to a decrease in the number of tandem rDNA repeats. Reintroduction of the missing gene induces an increase of rDNA repeats to reach a copy number similar to that of the original strain. Interestingly, while expansion is dependent on Fob1, a protein required for replication fork blocking activity in rDNA, contraction occurs in the absence of Fob1. Furthermore, silencing of class II genes at the rDNA, a process connected to rDNA stability, is not affected. Ctk1, the kinase subunit of the CTDK-I complex is involved in various steps of mRNA synthesis. In addition, we have recently shown that Ctk1 is also implicated in rRNA synthesis. The results suggest that the RNA polymerase I transcription defect occurring in a ctk1 mutant strain causes rDNA contraction.

Present addresses: Céline Bouchoux, Chromosome Segregation Laboratory, Cancer Research UK, 44 Lincoln's Inn fields, London WC2A 3PX, UK

Valérie Goguel, Gènes et Dynamique des Systèmes de Mémoire, CNRS UMR7637, ESPCI, 10 rue Vauquelin, 75005 Paris, France


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