Nucleic Acids Research Advance Access originally published online on September 20, 2006
Nucleic Acids Research 2006 34(18):5101-5111; doi:10.1093/nar/gkl671
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Nucleic Acids Research, 2006, Vol. 34, No. 18 5101-5111
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Genomics |
Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform
Genomics-Proteomics-Systemsbiology, Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik Nobelstraße 12, 70569 Stuttgart, Germany 1 Functional Genome Analysis Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580 69120 Heidelberg, Germany 2 Applied Biosystems Foster City, USA
*To whom correspondence should be addressed. Tel: +49 711 9704044; Fax: +49 711 9704200; Email: nicole.hauser{at}igb.fraunhofer.de
Received July 26, 2006. Revised August 29, 2006. Accepted September 1, 2006.
L-DNA is the perfect mirror-image form of the naturally occurring D-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA–protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.
The authors wish to be known that, in their opinion, the first two authors should be regarded as joint First Authors
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