Nucleic Acids Research Advance Access originally published online on September 29, 2006
Nucleic Acids Research 2006 34(19):5369-5382; doi:10.1093/nar/gkl612
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Nucleic Acids Research, 2006, Vol. 34, No. 19 5369-5382
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Molecular Biology |
Double-strand breaks in the myotonic dystrophy type 1 and the fragile X syndrome triplet repeat sequences induce different types of mutations in DNA flanking sequences in Escherichia coli
Center for Genome Research, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Texas Medical Center 2121 W. Holcombe Boulevard, Houston, TX 77030-3303, USA
*To whom correspondence should be addressed. Tel: +1 713 677 7651; Fax: +1 713 677 7689; Email: rwells{at}ibt.tamhsc.edu
Received May 2, 2006. Revised July 28, 2006. Accepted August 7, 2006.
The putative role of double-strand breaks (DSBs) created in vitro by restriction enzyme cleavage in or near CGG•CCG or CTG•CAG repeat tracts on their genetic instabilities, both within the repeats and in their flanking sequences, was investigated in an Escherichia coli plasmid system. DSBs at TRS junctions with the vector generated a large number of mutagenic events in flanking sequences whereas DSBs within the repeats elicited no similar products. A substantial enhancement in the number of mutants was caused by transcription of the repeats and by the absence of recombination functions (recA–, recBC–). Surprisingly, DNA sequence analyses on mutant clones revealed the presence of only single deletions of 0.4–1.6 kb including the TRS and the flanking sequence from plasmids originally containing (CGG•CCG)43 but single, double and multiple deletions as well as insertions were found for plasmids originally containing (CTG•CAG)n (where n = 43 or 70). Non-B DNA structures (slipped structures with loops, cruciforms, triplexes and tetraplexes) as well as microhomologies are postulated to participate in the recombination and/or repair processes.
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