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Nucleic Acids Research Advance Access originally published online on October 4, 2006
Nucleic Acids Research 2006 34(19):e131; doi:10.1093/nar/gkl713
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Nucleic Acids Research, 2006, Vol. 34, No. 19 e131
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Assessing incomplete deprotection of microarray oligonucleotides in situ

Holly K. Dressman, Lise Barley-Maloney1, Laura-Leigh Rowlette, Paul F. Agris1,* and Mariano A. Garcia-Blanco2,*

Center for Genome Technology, Institute for Genome Science and Policy Duke University, Durham, NC, USA 1 Department of Molecular and Structural Biochemistry, North Carolina State University Raleigh, NC, USA 2 Veri-Q, Inc. Raleigh NC, USA

*To whom correspondence should be addressed at Box 3053 DUMC, Research Drive, Durham, NC 27710, USA. Tel: +1 919 515 6188; Fax + 1 919 515 2047; Email: mariano.garciablanco{at}verizon.net

Received July 5, 2006. Revised September 7, 2006. Accepted September 13, 2006.

En masse analysis of gene structure and function by array technologies will have a lasting and profound effect on biology and medicine. This impact can be compromised by low quality of probes within arrays, which we show can be caused by incomplete removal of chemical protecting groups. To solve this quality control problem, we present a sensitive, specific and facile method to detect these groups in situ on arrays using monoclonal antibodies and existing instrumentation. Screening of microarrays with these monoclonal antibodies should guide the consideration given to data derived from these and should enhance the accuracy of the results obtained.

Correspondence may also be addressed to Paul F. Agris. Tel: +1 919 515 6188; Fax: +1 919 515 2047; Email: paul_agris{at}ncsu.edu


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