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Nucleic Acids Research 2006 34(2):436-444; doi:10.1093/nar/gkj451
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Published online 18 January 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
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Article

Sub-plastidial localization of two different phage-type RNA polymerases in spinach chloroplasts

Jacinthe Azevedo, Florence Courtois and Silva Lerbs-Mache*

Laboratoire Plastes et différenciation cellulaire, Université Joseph Fourier and Centre National de la Recherche Scientifique B.P. 53, 38041 Grenoble, France

*To whom correspondence should be addressed. Tel: +33 04 76 63 57 44; Fax: +33 04 76 63 55 86; Email: silva.lerbs-mache{at}ujf-grenoble.fr

Received November 23, 2005. Revised December 23, 2005. Accepted January 3, 2005.

Plant plastids contain a circular genome of ~150 kb organized into ~35 transcription units. The plastid genome is organized into nucleoids and attached to plastid membranes. This relatively small genome is transcribed by at least two different RNA polymerases, one being of the prokaryotic type and plastid-encoded (PEP), the other one being of the phage-type and nucleus-encoded (NEP). The presumed localization of a second phage-type RNA polymerase in plastids is still questionable. There is strong evidence for a sequential action of NEP and PEP enzymes during plant development attributing a prevailing role of NEP during early plant and plastid development, although NEP is present in mature chloroplasts. In the present paper, we have analysed two different NEP enzymes from spinach with respect to subcellular and intra-plastidial localization in mature chloroplasts with the help of specific antibodies. Results show the presence of the two different NEP enzymes in mature chloroplasts. Both enzymes are entirely membrane bound but, unlike previously thought, this membrane binding is not mediated via DNA. This finding indicates that NEP enzymes are not found as elongating transcription complexes on the template DNA in mature chloroplasts and raises the question of their function in mature chloroplasts.


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