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Nucleic Acids Research 2006 34(3):976-985; doi:10.1093/nar/gkj501
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Published online 9 February 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene

J. A. Ferreiro, N. G. Powell1, N. Karabetsou2, J. Mellor2 and R. Waters3,*

Department of Functional Biology, University of Oviedo Oviedo 33006, Spain 1Department of Obstetrics and Gynaecology, Medical School, Cardiff University Cardiff CF14 4XN, UK 2Department of Biochemistry, Oxford University Oxford OX1 3QU, UK 3Department of Pathology, Medical School, Cardiff University Cardiff CF14 4XN, UK

*To whom correspondence should be addressed. Tel: +44 2920744848; Fax: +44 2920744276; Email: watersr1{at}cf.ac.uk

Received November 25, 2005. Revised January 25, 2006. Accepted January 25, 2006.

Chromatin structure, transcription and repair of cyclobutane pyrimidine dimers at the MET16 gene of wild type, gcn5{Delta} and ada2{Delta} Saccharomyces cerevisiae cells were studied under repressing or derepressing conditions. These two components of the SAGA/ADA chromatin remodelling complexes are expendable for the basal transcription of MET16 but are mandatory for its full transcription induction. Despite their influence on transcription neither protein induces major changes in MET16 chromatin structure, but some minor ones occur. Repair at the coding region of the transcribed strand is faster than repair at non-transcribed regions in all strains and either growth condition. Moreover, the more MET16 is transcribed the faster the repair. The data show that by changing the transcription extent the rate of repair at each DNA strand is altered in a different way, confirming that repair at this locus is strongly modulated by its chromatin structure and transcription level. Deletion of GCN5 or ADA2 reduces repair at MET16. The results are discussed in light of the current understanding of Gcn5p and Ada2p functions, and they are the first to report a role for Ada2p in the nucleotide excision repair of the regulatory and transcribed regions of a gene.


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