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Nucleic Acids Research 2006 34(3):e20; doi:10.1093/nar/gnj019
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Published online 9 February 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

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Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

Gholam Khodakaramian, Sarah Lissenden, Bertolt Gust1, Laura Moir2, Paul A. Hoskisson2, Keith F. Chater1 and Margaret C. M. Smith2,*

Institute of Genetics, University of Nottingham Nottingham NG7 2UH, UK 1John Innes Centre, Norwich Research Park Colney Lane, Norwich NR4 7UH, UK 2Institute of Medical Sciences, University of Aberdeen Foresterhill, Aberdeen AB25 2ZD, UK

*To whom correspondence should be addressed. Tel: +44 1224 555739; Fax: + 44 1224 555844; Email: Maggie.smith{at}abdn.ac.uk

Received October 11, 2005. Revised December 12, 2005. Accepted January 19, 2006.

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage {varphi}C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

Present addresses: Gholam Khodakaramian, Department of Plant Protection, Abourayhan Campus, Tehran University, PO Box 11365/4117 (Pakdasht), Tehran, Iran

Bertolt Gust, Institute of Pharmacy, University of Tuebingen, Auf der Morgenstelle 8, 72076 Tuebingen, Germany


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M. Leibig, B. Krismer, M. Kolb, A. Friede, F. Gotz, and R. Bertram
Marker Removal in Staphylococci via Cre Recombinase and Different lox Sites
Appl. Envir. Microbiol., March 1, 2008; 74(5): 1316 - 1323.
[Abstract] [Full Text] [PDF]


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