Skip Navigation

Nucleic Acids Research 2006 34(5):1470-1480; doi:10.1093/nar/gkl023
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (2756K) Freely available
Right arrow Screen PDF (1104K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ramírez-Romero, M. A.
Right arrow Articles by Dávila, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramírez-Romero, M. A.
Right arrow Articles by Dávila, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 9 March 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Article

The Rhizobium etli {sigma}70 (SigA) factor recognizes a lax consensus promoter

Miguel A. Ramírez-Romero*, Irina Masulis, Miguel A. Cevallos, Víctor González and Guillermo Dávila

Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México Apartado Postal 565-A, C.P 62210, Cuernavaca, Morelos, México

*To whom correspondence should be addressed. Tel: +52 777 329 16 90; Fax: +52 777 317 55 81; Email: mramirez{at}ccg.unam.mx

Received December 2, 2005. Revised February 15, 2006. Accepted February 15, 2006.

A collection of Rhizobium etli promoters was isolated from a genomic DNA library constructed in the promoter-trap vector pBBMCS53, by their ability to drive the expression of a gusA reporter gene. Thirty-seven clones were selected, and their transcriptional start-sites were determined. The upstream sequence of these 37 start-sites, and the sequences of seven previously identified promoters were compared. On the basis of sequence conservation and mutational analysis, a consensus sequence CTTGACN16–23TATNNT was obtained. In this consensus sequence, nine on of twelve bases are identical to the canonical Escherichia coli {sigma}70 promoter, however the R.etli promoters only contain 6.4 conserved bases on average. We show that the R.etli sigma factor SigA recognizes all R.etli promoters studied in this work, and that E.coli RpoD is incapable of recognizing them. The comparison of the predicted structure of SigA with the known structure of RpoD indicated that regions 2.4 and 4.2, responsible for promoter recognition, are different only by a single amino acid, whereas the region 1 of SigA contains 72 extra residues, suggesting that the differences contained in this region could be related to the lax promoter recognition of SigA.

Correspondence may also be addressed to Guillermo Dávila. Tel: +52 777 329 16 90; Fax: 52 777 317 55 81; Email: davila{at}ccg.unam.mx


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
S. R. Khan and S. K. Farrand
The BlcC (AttM) Lactonase of Agrobacterium tumefaciens Does Not Quench the Quorum-Sensing System That Regulates Ti Plasmid Conjugative Transfer
J. Bacteriol., February 15, 2009; 191(4): 1320 - 1329.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. M. Martinez-Salazar, M. Sandoval-Calderon, X. Guo, S. Castillo-Ramirez, A. Reyes, M. G. Loza, J. Rivera, X. Alvarado-Affantranger, F. Sanchez, V. Gonzalez, et al.
The Rhizobium etli RpoH1 and RpoH2 sigma factors are involved in different stress responses
Microbiology, February 1, 2009; 155(2): 386 - 397.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. Bongiorni, T. Fukushima, A. C. Wilson, C. Chiang, M. C. Mansilla, J. A. Hoch, and M. Perego
Dual Promoters Control Expression of the Bacillus anthracis Virulence Factor AtxA
J. Bacteriol., October 1, 2008; 190(19): 6483 - 6492.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
E. Sepulveda, D. Perez-Mendoza, M. A. Ramirez-Romero, M. J. Soto, I. M. Lopez-Lara, O. Geiger, J. Sanjuan, S. Brom, and D. Romero
Transcriptional Interference and Repression Modulate the Conjugative Ability of the Symbiotic Plasmid of Rhizobium etli
J. Bacteriol., June 15, 2008; 190(12): 4189 - 4197.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
C. I. Muglia, D. H. Grasso, and O. M. Aguilar
Rhizobium tropici response to acidity involves activation of glutathione synthesis
Microbiology, April 1, 2007; 153(4): 1286 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Hadjifrangiskou, Y. Chen, and T. M. Koehler
The Alternative Sigma Factor {sigma}H Is Required for Toxin Gene Expression by Bacillus anthracis
J. Bacteriol., March 1, 2007; 189(5): 1874 - 1883.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.