Skip Navigation

Nucleic Acids Research 2006 34(6):1676-1684; doi:10.1093/nar/gkl090
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (664K) Freely available
Right arrow Screen PDF (674K) Freely available
Right arrow A corrigendum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Schimanski, B.
Right arrow Articles by Günzl, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schimanski, B.
Right arrow Articles by Günzl, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 22 March 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Article

A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei

Bernd Schimanski1, Jens Brandenburg1, Tu Ngoc Nguyen1, Melissa Jo Caimano2 and Arthur Günzl1,3,*

1Department of Genetics and Developmental Biology, University of Connecticut Health Center Farmington, CT 06030, USA 2Department of Medicine, University of Connecticut Health Center Farmington, CT 06030, USA 3Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center Farmington, CT 06030, USA

*To whom correspondence should be addressed. Tel: +1 860 679 8878; Fax: +1 860 679 8345; Email: gunzl{at}uchc.edu

Received February 7, 2006. Revised March 7, 2006. Accepted March 7, 2006.

The lack of general class II transcription factors was a hallmark of the genomic sequences of the human parasites Trypanosoma brucei, Trypanosoma cruzi and Leishmania major. However, the recent identification of TFIIA as part of a protein complex essential for RNA polymerase II-mediated transcription of SLRNA genes, which encode the trans splicing-specific spliced leader RNA, suggests that trypanosomatids assemble a highly divergent set of these factors at the SLRNA promoter. Here we report the identification of a trypanosomatid TFIIB-like (TFIIBlike) protein which has limited overall sequence homology to eukaryotic TFIIB and archaeal TFB but harbors conserved residues within the N-terminal zinc ribbon domain, the B finger and cyclin repeat I. In accordance with the function of TFIIB, T.brucei TFIIBlike is encoded by an essential gene, localizes to the nucleus, specifically binds to the SLRNA promoter, interacts with RNA polymerase II, and is absolutely required for SLRNA transcription.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
B. S. Ibrahim, N. Kanneganti, G. E. Rieckhof, A. Das, D. V. Laurents, J. B. Palenchar, V. Bellofatto, and D. A. Wah
Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei
PNAS, August 11, 2009; 106(32): 13242 - 13247.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Z. Palfi, N. Jae, C. Preusser, K. H. Kaminska, J. M. Bujnicki, J. H. Lee, A. Gunzl, C. Kambach, H. Urlaub, and A. Bindereif
SMN-assisted assembly of snRNP-specific Sm cores in trypanosomes
Genes & Dev., July 15, 2009; 23(14): 1650 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. H. Lee, H. S. Jung, and A. Gunzl
Transcriptionally active TFIIH of the early-diverged eukaryote Trypanosoma brucei harbors two novel core subunits but not a cyclin-activating kinase complex
Nucleic Acids Res., June 1, 2009; 37(11): 3811 - 3820.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Das, M. Banday, and V. Bellofatto
RNA Polymerase Transcription Machinery in Trypanosomes
Eukaryot. Cell, March 1, 2008; 7(3): 429 - 434.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. N. Nguyen, B. Schimanski, and A. Gunzl
Active RNA Polymerase I of Trypanosoma brucei Harbors a Novel Subunit Essential for Transcription
Mol. Cell. Biol., September 1, 2007; 27(17): 6254 - 6263.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. H. Lee, T. N. Nguyen, B. Schimanski, and A. Gunzl
Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIH
Eukaryot. Cell, April 1, 2007; 6(4): 641 - 649.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.