Published online 8 May 2006
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Oligoribonuclease is a common downstream target of lithium-induced pAp accumulation in Escherichia coli and human cells
Institut Pasteur, URA 2171, Unite de Génétique des Génomes Bactériens 75724 Paris Cedex 15, France 1 Institut Gustave Roussy, UMR 8126, Unite d' Interactions Moléculaires et Cancer 94805 Villejuif, France
*To whom correspondence should be addressed. Tel: +33 140613870; Fax: +33 145688948; Email: umechold{at}pasteur.fr
Received February 28, 2006. Revised March 12, 2006. Accepted March 29, 2006.
We identified Oligoribonuclease (Orn), an essential Escherichia coli protein and the only exonuclease degrading small ribonucleotides (5mer to 2mer) and its human homologue, small fragment nuclease (Sfn), in a screen for proteins that are potentially regulated by 3'-phosphoadenosine 5'-phosphate (pAp). We show that both enzymes are sensitive to micromolar amounts of pAp in vitro. We also demonstrate that Orn can degrade short DNA oligos in addition to its activity on RNA oligos, similar to what was documented for Sfn. pAp was shown to accumulate as a result of inhibition of the pAp-degrading enzyme by lithium, widely used to treat bipolar disorder, thus its regulatory targets are of significant medical interest. CysQ, the E.coli pAp-phosphatase is strongly inhibited by lithium and calcium in vitro and is a main target of lithium toxicity in vivo. Our findings point to remarkable conservation of the connection between sulfur- and RNA metabolism between E.coli and humans.
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