Skip Navigation

Nucleic Acids Research 2006 34(8):e58; doi:10.1093/nar/gkl160
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1128K) Freely available
Right arrow Screen PDF (1141K) Freely available
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hamilton, G.
Right arrow Articles by Pinkel, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamilton, G.
Right arrow Articles by Pinkel, D.
Related Collections
Right arrow Microarray
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 2 May 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Methods Online

A large field CCD system for quantitative imaging of microarrays

G. Hamilton1, N. Brown1, V. Oseroff1, B. Huey1,5, R. Segraves1,5, D. Sudar2, J. Kumler3, D. Albertson1,4,5 and D. Pinkel1,5,*

1 Comprehensive Cancer Center, University of California San Francisco CA, USA 2 Lawrence Berkeley National Laboratory Berkeley, CA, USA 3 Coastal Optical Systems West Palm Beach, FL, USA 4 Cancer Research Institute, University of California San Francisco CA, USA 5 Department of Laboratory Medicine, University of California San Francisco CA, USA

*To whom correspondence should be addressed. Tel: +1 415 476 3659; Fax: +1 415 476 8218; Email: pinkel{at}cc.ucsf.edu

Received February 13, 2006. Revised March 10, 2006. Accepted March 20, 2006.

We describe a charge-coupled device (CCD) imaging system for microarrays capable of acquiring quantitative, high dynamic range images of very large fields. Illumination is supplied by an arc lamp, and filters are used to define excitation and emission bands. The system is linear down to fluorochrome densities <<1 molecule/µm2. The ratios of the illumination intensity distributions for all excitation wavelengths have a maximum deviation ~±4% over the object field, so that images can be analyzed without computational corrections for the illumination pattern unless higher accuracy is desired. Custom designed detection optics produce achromatic images of the spectral region from ~ 450 to ~750 nm. Acquisition of a series of images of multiple fluorochromes from multiple arrays occurs under computer control. The version of the system described in detail provides images of 20 mm square areas using a 27 mm square, 2K x 2K pixel, cooled CCD chip with a well depth of ~105 electrons, and provides ratio measurements accurate to a few percent over a dynamic range in intensity >1000. Resolution referred to the sample is 10 µm, sufficient for obtaining quantitative multicolor images from >30 000 array elements in an 18 mm x 18 mm square.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Biomol ScreenHome page
S. M. Sukumaran, B. Potsaid, M.-Y. Lee, D. S. Clark, and J. S. Dordick
Development of a Fluorescence-Based, Ultra High-Throughput Screening Platform for Nanoliter-Scale Cytochrome P450 Microarrays
J Biomol Screen, July 1, 2009; 14(6): 668 - 678.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.