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The TIGR Maize Database
1The Institute for Genomic Research 9712 Medical Center Drive, Rockville, MD 20850, USA 2Department of Genetics, University of Georgia Athens, GA 30602, USA 3Genomics Center, Purdue University West Lafayette, IN 47906, USA 4Department of Biological Sciences, Purdue University West Lafayette, IN 47906, USA 5Donald Danforth Plant Science Center 975 North Warson Road, St Louis, MO 63132, USA 6Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute 44 Binney Street, Boston, MA 02115, USA 7Department of Biostatistics, Harvard School of Public Health Boston, MA 02115, USA
*To whom correspondence should be addressed. Tel: +1 301 795 7787; Fax: +1 301 838 0208; Email: pablo{at}tigr.org
Received August 15, 2005. Revised October 15, 2005. Accepted October 15, 2005.
Maize is a staple crop of the grass family and also an excellent model for plant genetics. Owing to the large size and repetitiveness of its genome, we previously investigated two approaches to accelerate gene discovery and genome analysis in maize: methylation filtration and high C0t selection. These techniques allow the construction of gene-enriched genomic libraries by minimizing repeat sequences due to either their methylation status or their copy number, yielding a 7-fold enrichment in genic sequences relative to a random genomic library. Approximately 900 000 gene-enriched reads from maize were generated and clustered into Assembled Zea mays (AZM) sequences. Here we report the current AZM release, which consists of 
298 Mb representing 243 807 sequence assemblies and singletons. In order to provide a repository of publicly available maize genomic sequences, we have created the TIGR Maize Database (http://maize.tigr.org). In this resource, we have assembled and annotated the AZMs and used available sequenced markers to anchor AZMs to maize chromosomes. We have constructed a maize repeat database and generated draft sequence assemblies of 287 maize bacterial artificial chromosome (BAC) clone sequences, which we annotated along with 172 additional publicly available BAC clones. All sequences, assemblies and annotations are available at the project website via web interfaces and FTP downloads.
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